{"version":"6.9","hitCount":18918,"nextCursorMark":"AoIIQKk1vSg1NTcxMjM1MA==","nextPageUrl":"https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=(canola OR rapeseed) AND (nitrogen OR drought OR seeding)&cursorMark=AoIIQKk1vSg1NTcxMjM1MA==&resultType=core&pageSize=30&format=json","request":{"queryString":"(canola OR rapeseed) AND (nitrogen OR drought OR seeding)","resultType":"core","cursorMark":"*","pageSize":30,"sort":"","synonym":false},"resultList":{"result":[{"id":"PMC13243059","source":"PMC","pmcid":"PMC13243059","fullTextIdList":{"fullTextId":["PMC13243059"]},"title":"Rhizosphere microbiome dynamics and plant adaptation to abiotic stress in major oilseed crops: a review","authorString":"Daurova A, Daurov D, Sapakhova Z, Kanat R, Abilda Z, Toishimanov M, Isgandarov I, Mukhametov A, Volkov D, Shamekova M, Zhambakin K.","authorList":{"author":[{"fullName":"Daurova 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J","firstName":"Jamshid","lastName":"Fooladi","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran."}]}},{"fullName":"Fazeli-Nasab B","firstName":"Bahman","lastName":"Fazeli-Nasab","initials":"B","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran. Fazelinsb@rizabol.ac.ir."}]}}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"1","volume":"26","journalIssueId":4187651,"dateOfPublication":"2026 May","monthOfPublication":5,"yearOfPublication":2026,"printPublicationDate":"2026-05-01","journal":{"title":"BMC plant biology","medlineAbbreviation":"BMC Plant Biol","isoabbreviation":"BMC Plant Biol","nlmid":"100967807","essn":"1471-2229","issn":"1471-2229"}},"pubYear":"2026","pageInfo":"896","abstractText":"The samples were under the influence of the treatment with pyrrolizine. Salinity adversely impacts oilseed production by leading to excessive salt accumulation and degrading soil structure. In rapeseed, salinity specifically hinders germination and the growth of aerial organs while also reducing the production of flavonoids and anthocyanins. Recent research has demonstrated that paclobutrazol treatment can enhance cellular activities under environmental stress. Accordingly, this study investigated the effects of paclobutrazol on the molecular mechanisms related to the synthesis of flavonoids, as one of the most important natural polyphenolic compounds. In this study, after applying salinity stress, the samples were under the influence of the treatment with paclobutrazol. The results of the expression evaluation of flavonoid synthesis-related genes production (MYB111 and MYB12) showed that paclobutrazol treatment had a significant effect on increasing the expression of these genes. The findings of this study also indicated that paclobutrazol treatment, in addition to reducing the negative effects of salinity, significantly increased the production of anthocyanins and the growth of plant aerial organs. MDA measures membrane damage from stress. SOD and POD are key antioxidant enzymes that protect plant cells from oxidative harm. Bioinformatics analyses of this study also showed that MYB111 and MYB12 genes have negative hydropathicity while interacting with a network of genes and transcription factors. These findings indicate that these genes, in addition to creating resistance to salinity stress, are also effective in reducing the effects of drought stress. This study's results indicate that applying paclobutrazol to oilseeds effectively prevents damage from salinity stress and reduces sodium ion accumulation in the plant structure.","affiliation":"Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran. amirzaei25@gmail.com.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug 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Germination and Flowering Stages.","authorString":"Daurova A, Daurov D, Sapakhova Z, Toishimanov M, Abilda Z, Kanat R, Shamekova M, Oshergina I, Ten E, Zhambakin K.","authorList":{"author":[{"fullName":"Daurova A","firstName":"Ainash","lastName":"Daurova","initials":"A","authorId":{"type":"ORCID","value":"0000-0001-7949-9112"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty 050040, Kazakhstan."},{"affiliation":"Faculty of Agrobiology, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan."}]}},{"fullName":"Daurov D","firstName":"Dias","lastName":"Daurov","initials":"D","authorId":{"type":"ORCID","value":"0000-0003-3073-4577"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty 050040, Kazakhstan."}]}},{"fullName":"Sapakhova Z","firstName":"Zagipa","lastName":"Sapakhova","initials":"Z","authorId":{"type":"ORCID","value":"0000-0002-8007-5066"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty 050040, Kazakhstan."},{"affiliation":"Tanir Research Laboratory, Almaty 050060, Kazakhstan."}]}},{"fullName":"Toishimanov M","firstName":"Maxat","lastName":"Toishimanov","initials":"M","authorId":{"type":"ORCID","value":"0000-0002-6070-4574"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty 050040, Kazakhstan."}]}},{"fullName":"Abilda 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I","firstName":"Irina","lastName":"Oshergina","initials":"I","authorId":{"type":"ORCID","value":"0000-0002-5131-5091"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"A.I. Barayev Research and Production Center for Grain Farming, Shortandy 021600, Kazakhstan."}]}},{"fullName":"Ten E","firstName":"Evgeniy","lastName":"Ten","initials":"E","authorId":{"type":"ORCID","value":"0000-0001-8173-672X"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"A.I. Barayev Research and Production Center for Grain Farming, Shortandy 021600, Kazakhstan."}]}},{"fullName":"Zhambakin K","firstName":"Kabyl","lastName":"Zhambakin","initials":"K","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty 050040, Kazakhstan."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-7949-9112"},{"type":"ORCID","value":"0000-0001-8173-672X"},{"type":"ORCID","value":"0000-0001-8505-5243"},{"type":"ORCID","value":"0000-0002-5131-5091"},{"type":"ORCID","value":"0000-0002-6070-4574"},{"type":"ORCID","value":"0000-0002-8007-5066"},{"type":"ORCID","value":"0000-0003-3073-4577"}]},"dataLinksTagsList":{"dataLinkstag":["altmetrics","supporting_data"]},"journalInfo":{"issue":"5","volume":"15","journalIssueId":4149034,"dateOfPublication":"2026 Feb","monthOfPublication":2,"yearOfPublication":2026,"printPublicationDate":"2026-02-01","journal":{"title":"Biology","medlineAbbreviation":"Biology (Basel)","isoabbreviation":"Biology (Basel)","nlmid":"101587988","essn":"2079-7737","issn":"2079-7737"}},"pubYear":"2026","pageInfo":"384","abstractText":"Drought stress is a major limiting factor for canola production in arid and semi-arid regions, particularly during seed germination, seedling and flowering stages. In this study, we evaluated drought responses of doubled haploid (DH) lines derived from interspecific hybrids of <i>B. napus</i> × <i>B. rapa</i> and their parental cultivars under simulated (PEG-6000) and soil-based drought conditions. Drought stress significantly reduced germination, growth, and physiological performance in all genotypes; however, DH lines consistently exhibited superior tolerance. Under PEG-induced osmotic stress, DH lines maintained higher germination rates, root elongation, and relative water content compared with parental genotypes. During seedling and flowering stages drought, DH lines showed lower accumulation of hydrogen peroxide and malondialdehyde, alongside markedly higher antioxidant enzyme activities (CAT and POD) and improved photosynthetic efficiency (Fv/Fm). Gene expression analysis revealed strong induction of drought-responsive genes, including <i>WRKY28</i>, <i>MYB</i>, <i>LTP</i>, <i>WSP</i>, <i>metallothionein</i>, and protein kinase family genes, particularly in DH lines at prolonged stress exposure. Multivariate analyses (PCA and correlation) confirmed a close association between enhanced antioxidant capacity, transcriptional activation, and drought tolerance traits. Overall, our results demonstrate that homozygous doubled haploid lines derived from distant hybridization between <i>B. napus</i> and <i>B. rapa</i> exhibit enhanced drought tolerance at both early and reproductive stages. These genotypes represent valuable genetic resources for breeding drought-tolerance canola cultivars.","affiliation":"Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty 050040, Kazakhstan.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"grantsList":{"grant":[{"grantId":"АР26102771","agency":"Ministry of Science and Higher Education of the Republic of Kazakhstan","orderIn":0}]},"keywordList":{"keyword":["PEG","Doubled haploid","Drought","Gene Expression","Canola"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/biology15050384"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12985168"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12985168?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"Y","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-03-13","dateOfCreation":"2026-03-13","firstIndexDate":"2026-03-14","fullTextReceivedDate":"2026-03-14","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-02-26","firstPublicationDate":"2026-02-26"},{"id":"41449218","source":"MED","pmid":"41449218","pmcid":"PMC12808777","fullTextIdList":{"fullTextId":["PMC12808777"]},"doi":"10.1038/s41598-025-31616-8","title":"Enhancing rapeseed germination by nano zinc oxide and zinc sulfate particles under interrupted irrigation.","authorString":"Alavifard SD, Taghvaei M, Naderi R, Edalat M, Heidari B, Dedicova B.","authorList":{"author":[{"fullName":"Alavifard SD","firstName":"Seyed Davood","lastName":"Alavifard","initials":"SD","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, School of Agriculture, Shiraz University, Shiraz, Iran."}]}},{"fullName":"Taghvaei M","firstName":"Mansour","lastName":"Taghvaei","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, School of Agriculture, Shiraz University, Shiraz, Iran. taghvaei@shirazu.ac.ir."}]}},{"fullName":"Naderi R","firstName":"Ruhollah","lastName":"Naderi","initials":"R","authorId":{"type":"ORCID","value":"0000-0003-2252-8124"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, School of Agriculture, Shiraz University, Shiraz, Iran."}]}},{"fullName":"Edalat M","firstName":"Mohsen","lastName":"Edalat","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, School of Agriculture, Shiraz University, Shiraz, Iran."}]}},{"fullName":"Heidari B","firstName":"Bahram","lastName":"Heidari","initials":"B","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, School of Agriculture, Shiraz University, Shiraz, Iran."}]}},{"fullName":"Dedicova B","firstName":"Beata","lastName":"Dedicova","initials":"B","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Breeding, Swedish University of Agricultural Sciences (SLU), Alnarp, Sweden. beata.dedicova@slu.se."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0003-2252-8124"}]},"journalInfo":{"issue":"1","volume":"16","journalIssueId":4083230,"dateOfPublication":"2025 Dec","monthOfPublication":12,"yearOfPublication":2025,"printPublicationDate":"2025-12-01","journal":{"title":"Scientific reports","medlineAbbreviation":"Sci Rep","isoabbreviation":"Sci Rep","nlmid":"101563288","essn":"2045-2322","issn":"2045-2322"}},"pubYear":"2025","pageInfo":"1994","abstractText":"The manuscript investigates the effect of zinc oxide nanoparticles and zinc sulfate (applied singly or in combination) on seed germination traits of four spring rapeseed cultivars, including three open-pollinated varieties (Delgan, Zafar, and RGS003) and the hybrid variety Hayola 50, under varying drought stress conditions imposed on the mother plants. The study employs a split-plot design over two years, assessing several germination and vigor indices. The results showed that application of zinc fertilizer in the form of a combination of foliar application of zinc sulfate and zinc oxide nanoparticles (at a concentration of 5 ppm) to the mother plant at three growth stages, namely germination, flowering, and pod formation, generally improves germination performance, especially under drought stress.","affiliation":"Department of Plant Production and Genetics, School of Agriculture, Shiraz University, Shiraz, Iran.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug effects","majorTopic_YN":"N"},{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Brassica rapa","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug effects","majorTopic_YN":"N"},{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Seeds","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug effects","majorTopic_YN":"N"},{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Zinc Oxide","meshQualifierList":{"meshQualifier":[{"abbreviation":"PD","qualifierName":"pharmacology","majorTopic_YN":"N"},{"abbreviation":"CH","qualifierName":"chemistry","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Zinc Sulfate","meshQualifierList":{"meshQualifier":[{"abbreviation":"PD","qualifierName":"pharmacology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Fertilizers"},{"majorTopic_YN":"Y","descriptorName":"Germination","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug effects","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Nanoparticles","meshQualifierList":{"meshQualifier":[{"abbreviation":"CH","qualifierName":"chemistry","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Droughts"},{"majorTopic_YN":"N","descriptorName":"Agricultural Irrigation"}]},"keywordList":{"keyword":["Germination","Brassica napus","Hybrid","Treatment","Nanoparticles"]},"chemicalList":{"chemical":[{"name":"Fertilizers","registryNumber":"0"},{"name":"Zinc Sulfate","registryNumber":"7733-02-0"},{"name":"Zinc Oxide","registryNumber":"SOI2LOH54Z"}]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1038/s41598-025-31616-8"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12808777"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12808777?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-01-16","dateOfCreation":"2025-12-25","firstIndexDate":"2025-12-27","fullTextReceivedDate":"2026-01-22","dateOfRevision":"2026-01-18","electronicPublicationDate":"2025-12-25","firstPublicationDate":"2025-12-25"},{"id":"PPR1157118","source":"PPR","doi":"10.21203/rs.3.rs-8756046/v1","title":"Foliar application of γ-aminobutyric acid (GABA) mitigates drought stress in canola (Brassica napus L.): Insights into antioxidant defense, oxidative stress, lipid profiling, and yield attributes","authorString":"Nayebi K, Shekari F, Abbasi A, Janmohammadi M, Sabaghnia N.","authorList":{"author":[{"fullName":"Nayebi K","firstName":"Khadijeh","lastName":"Nayebi","initials":"K"},{"fullName":"Shekari F","firstName":"Fariborz","lastName":"Shekari","initials":"F"},{"fullName":"Abbasi A","firstName":"Amin","lastName":"Abbasi","initials":"A"},{"fullName":"Janmohammadi M","firstName":"Mohsen","lastName":"Janmohammadi","initials":"M"},{"fullName":"Sabaghnia N","firstName":"Naser","lastName":"Sabaghnia","initials":"N"}]},"pubYear":"2026","abstractText":"<title>Abstract</title>  <p>  Water stress is a major environmental challenge that impacts plant growth and performance. Gamma-aminobutyric acid (GABA), an important regulatory molecule in metabolic pathways, plays a key role in plant growth and stress tolerance. This study aimed to examine the effect of foliar GABA treatment on antioxidant enzyme activity, physiological traits, growth measures, and both quantitative and qualitative yield of rapeseed (  <italic>Brassica napus</italic>  L.) under different water stress levels. The experiment was conducted over two consecutive years in a field setting, with three water stress levels (full irrigation, 60%, and 30% of field capacity) and four GABA concentrations (0, 25, 50, and 75 mM). GABA application, especially at 50 and 75 mM, boosted antioxidant enzyme activity and enhanced photosynthetic pigment content, while decreasing levels of proline, malondialdehyde, and hydrogen peroxide. The positive effects of GABA led to increased grain yield, Number of Siliques Per Plant, Number of Seeds Per Silique, and 1000 Kernel Weight. Additionally, GABA treatment raised the percentage, index, and oil yield compared to controls. Water stress reduced linolenic and linoleic fatty acids, while increasing oleic, erucic, and palmitic acids. However, GABA application at 50 and 75 mM alleviated these changes. Overall, the results suggest that GABA application can effectively enhance water stress tolerance and improve rapeseed yield by strengthening the antioxidant system and modulating physiological responses.  </p>","pubTypeList":{"pubType":["Preprint"]},"bookOrReportDetails":{"publisher":"Research Square","yearOfPublication":2026},"fullTextUrlList":{"fullTextUrl":[{"availability":"Free","availabilityCode":"F","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.21203/rs.3.rs-8756046/v1"}]},"commentCorrectionList":{"commentCorrection":[{"id":"42457871","source":"MED","reference":"Scientific reports. 2026 Jul;:","type":"Preprint of","note":"CrossRef Pre-print loader","orderIn":10001}]},"isOpenAccess":"N","inEPMC":"N","inPMC":"N","hasPDF":"N","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"N","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","versionList":{"version":[{"id":"PPR1157118","source":"PPR","firstPublishDate":"2026-02-20","versionNumber":1,"pubTypeList":{"pubType":["preprint"]},"hasEvaluations":"N"}]},"versionNumber":1,"hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCreation":"2026-02-21","firstIndexDate":"2026-02-21","firstPublicationDate":"2026-02-20"},{"id":"41255332","source":"MED","pmid":"41255332","pmcid":"PMC12628121","fullTextIdList":{"fullTextId":["PMC12628121"]},"doi":"10.1111/ppl.70644","title":"A Brief History of Canola Genetic Gains: From Classical Breeding to Genome Editing.","authorString":"Azhar M, Cahill DM, Khan GA.","authorList":{"author":[{"fullName":"Azhar M","firstName":"Maryam","lastName":"Azhar","initials":"M","authorId":{"type":"ORCID","value":"0009-0007-5059-6999"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life and Environmental Sciences & Centre for Sustainable Bioproducts, Deakin University, Waurn Ponds, Victoria, Australia."}]}},{"fullName":"Cahill DM","firstName":"David M","lastName":"Cahill","initials":"DM","authorId":{"type":"ORCID","value":"0000-0002-2556-0528"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life and Environmental Sciences & Centre for Sustainable Bioproducts, Deakin University, Waurn Ponds, Victoria, Australia."}]}},{"fullName":"Khan GA","firstName":"Ghazanfar Abbas","lastName":"Khan","initials":"GA","authorId":{"type":"ORCID","value":"0000-0001-5629-0682"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life and Environmental Sciences & Centre for Sustainable Bioproducts, Deakin University, Waurn Ponds, Victoria, Australia."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-5629-0682"},{"type":"ORCID","value":"0000-0002-2556-0528"},{"type":"ORCID","value":"0009-0007-5059-6999"}]},"journalInfo":{"issue":"6","volume":"177","journalIssueId":4037722,"dateOfPublication":"2025 Nov-Dec","monthOfPublication":0,"yearOfPublication":2025,"printPublicationDate":"2025-11-01","journal":{"title":"Physiologia plantarum","medlineAbbreviation":"Physiol Plant","isoabbreviation":"Physiol Plant","nlmid":"1256322","essn":"1399-3054","issn":"1399-3054"}},"pubYear":"2025","pageInfo":"e70644","abstractText":"Canola exemplifies the transformation of a crop from industrial use to a globally significant edible oilseed through sustained genetic and biotechnological innovation. Historically, rapeseed was characterized by high erucic acid and glucosinolate contents, restricting its use primarily to industrial applications. However, classical breeding efforts in the 1970s successfully developed 'double-low' canola varieties, significantly reducing erucic acid and glucosinolate levels, thus establishing canola as a safe and nutritious food-grade oil. Subsequent advancements, including the introduction of hybrid cultivars, markedly enhanced seed yields, while mutation breeding and marker-assisted selection refined key agronomic traits such as lodging tolerance and disease resistance. Later, biotechnology breakthroughs expanded canola's versatility, leading to specialty oils with tailored fatty-acid profiles, for example, high-laurate oils for industrial applications and omega-3 enriched oils for nutritional purposes, as well as herbicide-tolerant cultivars that simplified weed management. More recently, genome-editing technologies, notably CRISPR/Cas9, have accelerated trait improvement by precisely modifying oil composition and significantly enhancing pod shatter resistance. This review synthesizes major genetic and breeding milestones that collectively shaped modern canola, highlights ongoing challenges associated with its complex polyploid genome, and discusses how emerging approaches, including multi-omics integration, precision genome editing, and artificial intelligence, offer promising strategies to further enhance canola productivity and sustainability.","affiliation":"School of Life and Environmental Sciences & Centre for Sustainable Bioproducts, Deakin University, Waurn Ponds, Victoria, Australia.","publicationStatus":"ppublish","language":"eng","pubModel":"Print","pubTypeList":{"pubType":["Historical Article","review-article","Review","Journal Article"]},"grantsList":{"grant":[{"agency":"Australian Research Council","orderIn":0}]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Crops, Agricultural","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Genome, Plant"},{"majorTopic_YN":"N","descriptorName":"History, 20th Century"},{"majorTopic_YN":"N","descriptorName":"History, 21st Century"},{"majorTopic_YN":"Y","descriptorName":"Plant Breeding"},{"majorTopic_YN":"Y","descriptorName":"Gene Editing","meshQualifierList":{"meshQualifier":[{"abbreviation":"MT","qualifierName":"methods","majorTopic_YN":"N"}]}}]},"keywordList":{"keyword":["Breeding","Oil","Genetic gains","Canola"]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription 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by-nc-nd","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2025-11-19","dateOfCreation":"2025-11-19","firstIndexDate":"2025-11-19","fullTextReceivedDate":"2025-11-24","dateOfRevision":"2025-12-12","firstPublicationDate":"2025-11-01"},{"id":"41971542","source":"MED","pmid":"41971542","pmcid":"PMC13062686","fullTextIdList":{"fullTextId":["PMC13062686"]},"doi":"10.3389/fpls.2026.1752750","title":"Mitigation of soil water stress by moderately deep sowing and exogenous application of glucosinolates during the early seedling stage in rapeseed.","authorString":"Bai C, Lei Y, Batool M, El-Badri AM, Chang Y, Kuai J, Wang B, Zhao J, Xu Z, Anwar S, King GJ, Wang J, Zhou G.","authorList":{"author":[{"fullName":"Bai C","firstName":"Chenyang","lastName":"Bai","initials":"C","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Lei Y","firstName":"Yizhong","lastName":"Lei","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Batool M","firstName":"Maria","lastName":"Batool","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"El-Badri AM","firstName":"Ali Mahmoud","lastName":"El-Badri","initials":"AM","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."},{"affiliation":"Field Crops Research Institute, Agricultural Research Center (ARC), Giza, Egypt."}]}},{"fullName":"Chang Y","firstName":"Ying","lastName":"Chang","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Kuai J","firstName":"Jie","lastName":"Kuai","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Wang B","firstName":"Bo","lastName":"Wang","initials":"B","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Zhao J","firstName":"Jie","lastName":"Zhao","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Xu Z","firstName":"Zhenghua","lastName":"Xu","initials":"Z","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Anwar S","firstName":"Sumera","lastName":"Anwar","initials":"S","authorId":{"type":"ORCID","value":"0000-0001-8224-7370"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Botany, Government College Women University, Faisalabad, Pakistan."}]}},{"fullName":"King GJ","firstName":"Graham John","lastName":"King","initials":"GJ","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Southern Cross Plant Science, Southern Cross University, Lismore, NSW, Australia."}]}},{"fullName":"Wang J","firstName":"Jing","lastName":"Wang","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}},{"fullName":"Zhou G","firstName":"Guangsheng","lastName":"Zhou","initials":"G","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-8224-7370"}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"volume":"17","journalIssueId":4112080,"dateOfPublication":"2026 ","monthOfPublication":0,"yearOfPublication":2026,"printPublicationDate":"2026-01-01","journal":{"title":"Frontiers in plant science","medlineAbbreviation":"Front Plant Sci","isoabbreviation":"Front Plant Sci","nlmid":"101568200","essn":"1664-462X","issn":"1664-462X"}},"pubYear":"2026","pageInfo":"1752750","abstractText":"<h4>Introduction</h4>Determining the optimal sowing depth suitable for different water conditions is a key agronomic factor for crop establishment and yield potential. This study aimed to identify the optimal sowing depth for rapeseed that maximizes seedling vigor under varying water conditions.<h4>Methods</h4>Seedling emergence and plant growth were evaluated under four water conditions (variable moisture, drought, normal water, and waterlogged) at different sowing depths (1-5 cm). Meanwhile, the hypothesis that seedling vigor under deep sowing conditions could be improved by exogenous application of glucosinolates (GS) was tested.<h4>Results</h4>Results indicated that the highest seedling emergence percentage (EP) was observed at 3 cm, representing increases of 123.2% (variable moisture), 100% (drought), and 11.1% (normal water) compared with 1 cm. Under waterlogged stress, seedling EP showed no significant differences between 1 and 3 cm for 50% of the 16 cultivars. Moreover, seedling EP was significantly improved at 3 cm after seed priming with GS compared with 1 cm, with increases of 46.4% (drought) and 63.0% (waterlogged), whereas no significant differences were observed under normal water conditions. Furthermore, plant phenotypic performance indices were higher at 3 cm with GS treatment than at 1 cm across all water conditions.<h4>Discussion</h4>Collectively, a sowing depth of 3 cm combined with exogenous application of GS not only promoted seedling emergence but also benefited subsequent plant growth in direct-sown rapeseed. These results provide practical insights for ensuring reliable seedling establishment in rapeseed.","affiliation":"Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic-eCollection","pubTypeList":{"pubType":["research-article","Journal Article"]},"keywordList":{"keyword":["Abiotic stress","Seedling vigor","Sowing depth","Canola","Crop Establishment"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3389/fpls.2026.1752750"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13062686"},{"availability":"Open 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by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-04-13","dateOfCreation":"2026-04-13","firstIndexDate":"2026-04-13","fullTextReceivedDate":"2026-04-10","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-03-06","firstPublicationDate":"2026-03-06"},{"id":"41820847","source":"MED","pmid":"41820847","pmcid":"PMC13094089","fullTextIdList":{"fullTextId":["PMC13094089"]},"doi":"10.1186/s12870-026-08487-w","title":"Pan-genomic analysis and abiotic stress expression of eight TPS gene families in Brassica napus.","authorString":"Xue T, Liu Z, He H, Wan H, Dai X, Zeng C, Zhang X, Yin S.","authorList":{"author":[{"fullName":"Xue T","firstName":"Tianyuan","lastName":"Xue","initials":"T","authorId":{"type":"ORCID","value":"0000-0003-2127-6754"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China."}]}},{"fullName":"Liu Z","firstName":"Zixiang","lastName":"Liu","initials":"Z","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China."}]}},{"fullName":"He H","firstName":"Huachuan","lastName":"He","initials":"H","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China."}]}},{"fullName":"Wan H","firstName":"Heping","lastName":"Wan","initials":"H","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China."}]}},{"fullName":"Dai X","firstName":"Xigang","lastName":"Dai","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China."}]}},{"fullName":"Zeng C","firstName":"Changli","lastName":"Zeng","initials":"C","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China. zengchangli@jhun.edu.cn."}]}},{"fullName":"Zhang X","firstName":"Xiangxiang","lastName":"Zhang","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Wuhan, China. zhangxiangxiang@caas.cn."}]}},{"fullName":"Yin S","firstName":"Shuai","lastName":"Yin","initials":"S","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China. yinshuai@jhun.edu.cn."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0003-2127-6754"}]},"journalInfo":{"issue":"1","volume":"26","journalIssueId":4146486,"dateOfPublication":"2026 Mar","monthOfPublication":3,"yearOfPublication":2026,"printPublicationDate":"2026-03-01","journal":{"title":"BMC plant biology","medlineAbbreviation":"BMC Plant Biol","isoabbreviation":"BMC Plant Biol","nlmid":"100967807","essn":"1471-2229","issn":"1471-2229"}},"pubYear":"2026","pageInfo":"716","abstractText":"TPS transcription factors play crucial roles in plant growth, development, and responses to abiotic stress. In this study, we identified 90 BnTPS genes across the Brassica napus pan-genome comprising eight high-quality genomes, including 11 core, 2 near-core, 6 non-essential, and 3 private genes. Ka/Ks analysis revealed that BnTPS38, BnTPS41, and particularly BnTPS20 (frequently > 1), along with BnTPS19 (up to 11.38), showed strong signals of positive selection, suggesting adaptation during domestication. In contrast, BnTPS14 and BnTPS16 consistently exhibited Ka/Ks < 1, indicating purifying selection. Notably, BnTPS41 displayed moderate exon SNP variation causing missense mutations in the Gangan and Shengli cultivars, which correlated with distinct expression profiles. Expression analyses under different sowing stages and abiotic stresses showed that BnTPS17 and BnTPS45 maintained high expression in leaves across most stages and stresses except freezing, while BnTPS16 from the TPS-e subfamily was highly expressed in leaves and roots under stress and during various sowing stages (except in No2127). These findings deepen our understanding of the evolutionary dynamics and functional diversity of BnTPS genes and provide candidate genes for future genetic improvement of B. napus.","affiliation":"Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan, Hubei, 430056, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"grantsList":{"grant":[{"grantId":"2022ZD04010","agency":"National Major Project of Biological Breeding","orderIn":0},{"grantId":"2025JCYJ18","agency":"the Research Fund of Jianghan University","orderIn":0}]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"},{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Plant Proteins","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"},{"abbreviation":"ME","qualifierName":"metabolism","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Transcription Factors","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"},{"abbreviation":"ME","qualifierName":"metabolism","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Genomics"},{"majorTopic_YN":"N","descriptorName":"Phylogeny"},{"majorTopic_YN":"N","descriptorName":"Gene Expression Regulation, Plant"},{"majorTopic_YN":"Y","descriptorName":"Genes, Plant"},{"majorTopic_YN":"Y","descriptorName":"Genome, Plant"},{"majorTopic_YN":"N","descriptorName":"Multigene Family"},{"majorTopic_YN":"Y","descriptorName":"Stress, Physiological","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"}]}}]},"keywordList":{"keyword":["Abiotic stress","TPS","Positive Selection","Snp Variation","Pan-genome"]},"chemicalList":{"chemical":[{"name":"Plant Proteins","registryNumber":"0"},{"name":"Transcription Factors","registryNumber":"0"}]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1186/s12870-026-08487-w"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13094089"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13094089?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"Y","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by-nc-nd","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-06-27","dateOfCreation":"2026-03-13","firstIndexDate":"2026-03-13","fullTextReceivedDate":"2026-04-22","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-03-12","firstPublicationDate":"2026-03-12"},{"id":"41672946","source":"MED","pmid":"41672946","pmcid":"PMC12915857","fullTextIdList":{"fullTextId":["PMC12915857"]},"doi":"10.1080/15592324.2026.2630126","title":"Synergistic application of melatonin and methyl jasmonate mitigates drought-induced oxidative and photosynthetic impairment in &lt;i&gt;Brassica napus&lt;/i&gt;.","authorString":"Amiri H, Hemmati Hassan Gavyar P.","authorList":{"author":[{"fullName":"Amiri H","firstName":"Hamzeh","lastName":"Amiri","initials":"H","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biology, Faculty of Basic Science, Lorestan University, Khorramabad, Iran."}]}},{"fullName":"Hemmati Hassan Gavyar P","firstName":"Parvaneh","lastName":"Hemmati Hassan Gavyar","initials":"P","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biology, Faculty of Basic Science, Lorestan University, Khorramabad, Iran."}]}}]},"journalInfo":{"issue":"1","volume":"21","journalIssueId":4076768,"dateOfPublication":"2026 Dec","monthOfPublication":12,"yearOfPublication":2026,"printPublicationDate":"2026-12-01","journal":{"title":"Plant signaling & behavior","medlineAbbreviation":"Plant Signal Behav","isoabbreviation":"Plant Signal Behav","nlmid":"101291431","essn":"1559-2324","issn":"1559-2316"}},"pubYear":"2026","pageInfo":"2630126","abstractText":"Drought stress severely limits rapeseed (Brassica napus L.) growth and productivity by disrupting photosynthetic efficiency and inducing oxidative damage. This study investigated the potential of melatonin (MT) and methyl jasmonate (MJ), individually and in combination, to mitigate drought-induced toxicity in rapeseed. Plants were subjected to drought stress (15% Polyethylene glycol 6000), followed by foliar application of MT (100 μM), MJ (100 μM), or their combination. Under drought stress, rapeseed exhibited significantly reduced chlorophyll content (by 33.72% for chl a and 54.59% for chl b), impaired photosystem II efficiency (Fv/Fm decreased by 16.08%), and elevated oxidative stress markers (H₂O₂ increased by 54.79%, MDA by 144.6%, ROS by 43.96%). The combined application of MT and MJ conferred the highest level of drought tolerance, increasing shoot fresh weight by 92.33% and root dry weight by 405% compared to the stressed control. This synergistic treatment effectively enhanced stomatal conductance by 76.25%, increased net photosynthetic rate (Pn) by 71.45%, and boosted key antioxidant enzyme activities (e.g., POD by 216%). The enhanced efficacy of the dual treatment compared to individual applications strongly indicates coordinated signaling between MT and MJ pathways in stress response modulation. These results demonstrate that simultaneous application of these plant growth regulators offers an effective physiological strategy to improve rapeseed performance under water deficit conditions.","affiliation":"Department of Biology, Faculty of Basic Science, Lorestan University, Khorramabad, Iran.","publicationStatus":"ppublish","language":"eng","pubModel":"Print-Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug effects","majorTopic_YN":"N"},{"abbreviation":"ME","qualifierName":"metabolism","majorTopic_YN":"N"},{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Reactive Oxygen 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Environmental Engineering University of Saskatchewan Saskatoon Saskatchewan Canada."},{"affiliation":"Global Institute for Water Security University of Saskatchewan Saskatoon Saskatchewan Canada."}]}},{"fullName":"Papalexiou SM","firstName":"Simon Michael","lastName":"Papalexiou","initials":"SM","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Civil, Geological, and Environmental Engineering University of Saskatchewan Saskatoon Saskatchewan Canada."},{"affiliation":"Global Institute for Water Security University of Saskatchewan Saskatoon Saskatchewan Canada."},{"affiliation":"Institute of Global Water Security Hamburg University of Technology Hamburg Germany."},{"affiliation":"Institute for Water, Environment and Health United Nations University Hamilton Ontario Canada."}]}},{"fullName":"Li Y","firstName":"Yanping","lastName":"Li","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Physics and Astronomy Western University London Ontario Canada."}]}},{"fullName":"Elshorbagy A","firstName":"Amin","lastName":"Elshorbagy","initials":"A","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Civil, Geological, and Environmental Engineering University of Saskatchewan Saskatoon Saskatchewan Canada."},{"affiliation":"Global Institute for Water Security University of Saskatchewan Saskatoon Saskatchewan Canada."}]}},{"fullName":"Schuster-Wallace C","firstName":"Corinne","lastName":"Schuster-Wallace","initials":"C","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Global Institute for Water Security University of Saskatchewan Saskatoon Saskatchewan Canada."},{"affiliation":"Department of Geography and Planning University of Saskatchewan Saskatoon Saskatchewan Canada."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-8658-4243"}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"1","volume":"7","journalIssueId":4081284,"dateOfPublication":"2026 Feb","monthOfPublication":2,"yearOfPublication":2026,"printPublicationDate":"2026-02-01","journal":{"title":"Plant-environment interactions (Hoboken, N.J.)","medlineAbbreviation":"Plant Environ Interact","isoabbreviation":"Plant Environ Interact","nlmid":"9918573880306676","essn":"2575-6265","issn":"2575-6265"}},"pubYear":"2026","pageInfo":"e70107","abstractText":"Alberta, Manitoba, and Saskatchewan, the Prairie Provinces of Canada, lead national oilseed cultivation. Canola is a staple crop that provides food oil and feedstock for biofuels. Canola production is vulnerable to climate variability, and climate change has altered crop cycles and affected Canadian canola producers. This study aims to generate an initial understanding of canola producers' perceptions of climate change, their current adaptation strategies, and drivers and barriers to implementing new adaptation strategies in the Canadian Prairies. Besides, identifying the public policy needs to improve the canola production sector. Data were collected through an online survey and key informant interviews. Most participants identified changes in climate and frequency of extreme events. They identified a higher occurrence of heat waves and wind gusts and had to adapt to a higher presence of pests and diseases. Despite climate variability, canola productivity has improved in the last ten years, attributed to better technology and management of inputs. Genetic improvement is seen as a crucial part of canola's resistance to biotic and abiotic events. Most participant producers make independent decisions regarding adaptation and best practices at the field level. There is a vast and diverse outreach from researchers and specialists that producers are able to use in decision-making around implementing new or improved technologies. Participants recommended new and enhanced public policies to regulate the canola industry and seed market, technologies and data use, fossil fuel use, land and water management, and crop nutrition. These initial understandings point to ways in which regulatory bodies and specialists can continue to support producers to mitigate negative impacts of a changing climate and inform shareholders and policymakers of current needs and expectations.","affiliation":"Department of Civil, Geological, and Environmental Engineering University of Saskatchewan Saskatoon Saskatchewan Canada.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic-eCollection","pubTypeList":{"pubType":["research-article","Journal Article"]},"grantsList":{"grant":[{"grantId":"RGPIN‐2019‐06894","agency":"Natural Sciences and Engineering Research Council of Canada","orderIn":0},{"grantId":"RGPIN‐2019‐04590","agency":"Natural Sciences and Engineering Research Council of Canada","orderIn":0}]},"keywordList":{"keyword":["Climate change","Perceptions","Beneficial Management Practices"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1002/pei3.70107"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12766075"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12766075?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"Y","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"Y","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-01-07","dateOfCreation":"2026-01-07","firstIndexDate":"2026-01-08","fullTextReceivedDate":"2026-01-07","dateOfRevision":"2026-01-09","electronicPublicationDate":"2026-01-04","firstPublicationDate":"2026-01-04"},{"id":"42457871","source":"MED","pmid":"42457871","doi":"10.1038/s41598-026-61961-1","title":"Foliar γ-aminobutyric acid enhances antioxidant defense and yield performance of canola under drought stress.","authorString":"Nayebi K, Shekari F, Abbasi A, Janmohammadi M, Sabaghnia N.","authorList":{"author":[{"fullName":"Nayebi K","firstName":"Khadijeh","lastName":"Nayebi","initials":"K","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran."}]}},{"fullName":"Shekari F","firstName":"Fariborz","lastName":"Shekari","initials":"F","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran. shekari@maragheh.ac.ir."}]}},{"fullName":"Abbasi A","firstName":"Amin","lastName":"Abbasi","initials":"A","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran."}]}},{"fullName":"Janmohammadi M","firstName":"Mohsen","lastName":"Janmohammadi","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran."}]}},{"fullName":"Sabaghnia N","firstName":"Naser","lastName":"Sabaghnia","initials":"N","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran."}]}}]},"journalInfo":{"journalIssueId":4219416,"dateOfPublication":"2026 Jul","monthOfPublication":7,"yearOfPublication":2026,"printPublicationDate":"2026-07-01","journal":{"title":"Scientific reports","medlineAbbreviation":"Sci Rep","isoabbreviation":"Sci Rep","nlmid":"101563288","essn":"2045-2322","issn":"2045-2322"}},"pubYear":"2026","abstractText":"Drought stress is one of the major constraints on canola productivity, affecting growth, yield, and oil quality. This two-year field study investigated the potential of foliar-applied γ-aminobutyric acid (GABA) to enhance drought tolerance by modulating antioxidant defenses and physiological responses. Canola plants were subjected to three irrigation regimes; non-stress, moderate (60% field capacity), and severe stress (30% field capacity), and foliar-sprayed with GABA (0, 25, 50, and 75 mM). Drought stress significantly impaired plant performance, reducing grain yield by up to 48%, thousand-kernel weight by 36%, and chlorophyll content by 28%, while increasing oxidative stress markers such as malondialdehyde (MDA) and hydrogen peroxide by 52% and 61%, respectively. Foliar application of GABA, particularly at 50 mM, markedly mitigated these effects: antioxidant enzyme activities (catalase, superoxide dismutase, ascorbate peroxidase, and guaiacol peroxidase) increased by 35-62%, proline accumulation decreased by up to 24%, and chlorophyll content recovered by 21-30% under stress conditions. GABA-treated plants exhibited enhanced growth, higher grain yield (up to 41% increase under moderate stress), and improved oil yield and quality, including maintenance of linolenic and linoleic acids and reduction of stress-induced oleic and palmitic acids. Correlation-based hierarchical cluster analysis showed that yield-related traits like seed number and pods per plant clustered together, while plant height and seed dimensions formed separate sub-clusters, highlighting key trait networks for selection. Principal component analysis indicated that 50 and 75 mM GABA treatments, particularly under water deficit, enhanced antioxidant activity and maintained yield-related traits, demonstrating their effectiveness in improving drought tolerance. These findings provide field-based evidence that foliar-applied GABA, particularly at 50-75 mM, can improve drought resilience, yield performance, and oil-related traits in canola. Thus, foliar application of 50-75 mM GABA offers a promising strategy to enhance drought resilience in canola, providing both physiological protection and economic benefits.","affiliation":"Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran.","publicationStatus":"aheadofprint","language":"eng","pubModel":"Print-Electronic","pubTypeList":{"pubType":["Journal Article"]},"keywordList":{"keyword":["Drought stress","Antioxidant enzymes","(Brassica Napus L.)","Fatty Acids Compounds","Tolerance To Abiotic Stresses"]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1038/s41598-026-61961-1"}]},"commentCorrectionList":{"commentCorrection":[{"id":"PPR1157118","source":"PPR","type":"Preprint in","note":"CrossRef Pre-print loader","orderIn":10002}]},"isOpenAccess":"N","inEPMC":"N","inPMC":"N","hasPDF":"N","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"N","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by-nc-nd","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCreation":"2026-07-15","firstIndexDate":"2026-07-16","dateOfRevision":"2026-07-15","electronicPublicationDate":"2026-07-15","firstPublicationDate":"2026-07-15"},{"id":"41829726","source":"MED","pmid":"41829726","pmcid":"PMC12986939","fullTextIdList":{"fullTextId":["PMC12986939"]},"doi":"10.3390/plants15050694","title":"BnaMYB73, a &lt;i&gt;Brassica napus&lt;/i&gt; L. R2R3-MYB Transcription Factor, Enhances Plant Salt and Osmotic Stress Tolerance.","authorString":"Wang L, Zhang Y, Zhou X, Xu X, Zhang H, Sun N, Li D, Liu Y.","authorList":{"author":[{"fullName":"Wang L","firstName":"Limin","lastName":"Wang","initials":"L","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."}]}},{"fullName":"Zhang Y","firstName":"Yuzhe","lastName":"Zhang","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."}]}},{"fullName":"Zhou X","firstName":"Xiaoyan","lastName":"Zhou","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."}]}},{"fullName":"Xu X","firstName":"Xin","lastName":"Xu","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."}]}},{"fullName":"Zhang H","firstName":"Hongxia","lastName":"Zhang","initials":"H","authorId":{"type":"ORCID","value":"0000-0002-4204-7179"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."},{"affiliation":"School of Biological Science and Technology, University of Jinan, 336 Nanxinzhuangxi Road, Jinan 250024, China."}]}},{"fullName":"Sun N","firstName":"Nan","lastName":"Sun","initials":"N","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."}]}},{"fullName":"Li D","firstName":"Dong","lastName":"Li","initials":"D","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."}]}},{"fullName":"Liu Y","firstName":"Yanfeng","lastName":"Liu","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China."},{"affiliation":"Yantai Key Laboratory of Crop Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation, College of Horticulture, Ludong University, Yantai 264025, China."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0002-4204-7179"}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"5","volume":"15","journalIssueId":4149950,"dateOfPublication":"2026 Feb","monthOfPublication":2,"yearOfPublication":2026,"printPublicationDate":"2026-02-01","journal":{"title":"Plants (Basel, Switzerland)","medlineAbbreviation":"Plants (Basel)","isoabbreviation":"Plants (Basel)","nlmid":"101596181","essn":"2223-7747","issn":"2223-7747"}},"pubYear":"2026","pageInfo":"694","abstractText":"MYB transcription factors (TFs) are crucial for plant growth, development, and response to abiotic stress. However, their exact functions in abiotic stress responses in rapeseed remain largely unexplored. In this study, we identified and characterized <i>BnaMYB73</i>, a member of the R2R3-MYB subfamily, and investigated its role in abiotic stress tolerance. The transcription level of <i>BnaMYB73</i> was significantly upregulated in response to salt and osmotic stress. Transgenic <i>Arabidopsis thaliana</i> lines expressing <i>BnaMYB73</i> displayed significantly enhanced tolerance to salt and osmotic stress, while showing no phenotypic differences in growth compared with wild-type (WT) plants under normal conditions. Physiological analyses revealed that the <i>BnaMYB73</i>-expressing plants accumulated higher proline levels, exhibited elevated superoxide dismutase (SOD) and peroxidase (POD) activities, and reduced malondialdehyde (MDA) content under stress conditions. Moreover, the <i>BnaMYB73</i>-expressing plants significantly upregulated key stress-responsive genes, including <i>AtRD29B</i>, <i>AtDREB2A</i>, <i>AtRAB18</i>, <i>AtP5CS1</i>, <i>AtSOS1</i> and <i>AtCAT1</i>. Collectively, these findings establish BnaMYB73 functions as a stress-responsive transcription factor that enhances abiotic stress tolerance and provide a promising target for breeding stress-resilient rapeseed cultivars.","affiliation":"Yantai Technology Center of Characteristic Plant Gene Editing and Germplasm Innovation, Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai 264025, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"grantsList":{"grant":[{"grantId":"32371915","agency":"National Natural Science Foundation of China","orderIn":0},{"grantId":"none","agency":"Double-Hundred Talents Project of Yantai City","orderIn":0},{"grantId":"32071733","agency":"National Natural Science Foundation of China","orderIn":0},{"grantId":"ZR2019PC015","agency":"Natural Science Foundation of Shandong Province, China","orderIn":0},{"grantId":"ZR2020QC115","agency":"Natural Science Foundation of Shandong Province, China","orderIn":0},{"grantId":"31901572","agency":"National Natural Science Foundation of China","orderIn":0},{"agency":"Double-Hundred Talents Project of Yantai City","orderIn":0}]},"keywordList":{"keyword":["Brassica napus L.","Transgenic Plants","Salt And Osmotic Stress","Bnamyb73 Transcription Factors"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/plants15050694"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12986939"},{"availability":"Open 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Progress.","authorString":"Zhou M, Deng W, Dai B, Yu Q, Zhou W, Zan X, Song X.","authorList":{"author":[{"fullName":"Zhou M","firstName":"Menglin","lastName":"Zhou","initials":"M","authorId":{"type":"ORCID","value":"0009-0004-2670-4775"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China."}]}},{"fullName":"Deng W","firstName":"Wuming","lastName":"Deng","initials":"W","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China."}]}},{"fullName":"Dai B","firstName":"Bingbing","lastName":"Dai","initials":"B","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China."}]}},{"fullName":"Yu Q","firstName":"Qingqing","lastName":"Yu","initials":"Q","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China."}]}},{"fullName":"Zhou W","firstName":"Wei","lastName":"Zhou","initials":"W","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China."}]}},{"fullName":"Zan X","firstName":"Xiaofei","lastName":"Zan","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China."}]}},{"fullName":"Song X","firstName":"Xi","lastName":"Song","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China."},{"affiliation":"College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0009-0004-2670-4775"}]},"journalInfo":{"issue":"9","volume":"47","journalIssueId":4022033,"dateOfPublication":"2025 Sep","monthOfPublication":9,"yearOfPublication":2025,"printPublicationDate":"2025-09-01","journal":{"title":"Current issues in molecular biology","medlineAbbreviation":"Curr Issues Mol Biol","isoabbreviation":"Curr Issues Mol Biol","nlmid":"100931761","essn":"1467-3045","issn":"1467-3037"}},"pubYear":"2025","pageInfo":"755","abstractText":"Silique dehiscence is a critical biological phenomenon in rapeseed production that significantly influences seed maturity, harvesting efficiency, and ultimately yield. As one of the world's most important oilseed crops, studying the mechanisms underlying silique dehiscence in rapeseed (<i>Brassica napus</i> L.) not only aids in understanding fundamental principles of plant development but also provides a scientific basis for optimizing agricultural production practices. Silique dehiscence occurs naturally during the maturation process of rapeseed, with the timing and extent of this phenomenon directly affecting seed harvesting efficiency. This paper reviews the research progress regarding the mechanization of canola production, which enhances harvesting efficiency by enabling timely harvest coordination to minimize pre-harvest shattering losses and reduce post-harvest seed damage. Additionally, it addresses the factors influencing pod shattering, the process of pod shattering, the genes associated with this phenomenon, and the molecular mechanisms underlying pod shattering. These findings establish a foundation for a comprehensive understanding of pod shattering in canola.","affiliation":"Nanchong Academy of Agricultural Sciences, Nanchong 637000, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"grantsList":{"grant":[{"grantId":"2022ZDZX0015","agency":"Sichuan Rape Innovation Team","orderIn":0},{"grantId":"2022ZDZX0015","agency":"Sichuan Science and Technology Program","orderIn":0},{"grantId":"QJZD2401","agency":"Sichuan Major Science and Technology Project","orderIn":0},{"grantId":"SCCXTD-2025-3","agency":"Sichuan Science and Technology Program","orderIn":0},{"grantId":"2021YFYZ0018","agency":"Nanchong Academy of Agricultural Sciences Youth Fund Key Project","orderIn":0},{"grantId":"2021YFYZ0018","agency":"Sichuan Science and Technology Program","orderIn":0},{"grantId":"2021YFYZ0018","agency":"Sichuan Major Science and Technology Project","orderIn":0},{"grantId":"2021YFYZ0018","agency":"Sichuan Rape Innovation Team","orderIn":0},{"grantId":"2022ZDZX0015","agency":"Sichuan Major Science and Technology Project","orderIn":0},{"grantId":"QJZD2401","agency":"Nanchong Academy of Agricultural Sciences Youth Fund Key Project","orderIn":0},{"grantId":"SCCXTD-2025-3","agency":"Nanchong Academy of Agricultural Sciences Youth Fund Key Project","orderIn":0},{"grantId":"2022ZDZX0015","agency":"Nanchong Academy of Agricultural Sciences Youth Fund Key Project","orderIn":0},{"grantId":"SCCXTD-2025-3","agency":"Sichuan Major Science and Technology Project","orderIn":0},{"grantId":"QJZD2401","agency":"Sichuan Rape Innovation Team","orderIn":0},{"grantId":"QJZD2401","agency":"Sichuan Science and Technology Program","orderIn":0},{"grantId":"SCCXTD-2025-3","agency":"Sichuan Rape Innovation Team","orderIn":0}]},"keywordList":{"keyword":["Genes","Molecular mechanism","Mechanization","rapeseed","Pod Dehiscence"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/cimb47090755"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12469059"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12469059?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2025-09-29","dateOfCreation":"2025-09-29","firstIndexDate":"2025-09-30","fullTextReceivedDate":"2025-10-01","dateOfRevision":"2025-10-02","electronicPublicationDate":"2025-09-12","firstPublicationDate":"2025-09-12"},{"id":"41463894","source":"MED","pmid":"41463894","pmcid":"PMC12729943","fullTextIdList":{"fullTextId":["PMC12729943"]},"doi":"10.3390/ani15243609","title":"Canola Meal: A Sustainable Protein Source for Poultry Diets.","authorString":"Dao TH, Moss AF.","authorList":{"author":[{"fullName":"Dao TH","firstName":"Thi Hiep","lastName":"Dao","initials":"TH","authorId":{"type":"ORCID","value":"0000-0002-3093-1207"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Environmental and Rural Science, Faculty of Science, Agriculture, Business and Law, University of New England, Armidale, NSW 2351, Australia."},{"affiliation":"Faculty of Animal Science, Vietnam National University of Agriculture, Ngo Xuan Quang Street, Gia Lam Commune, Hanoi 100000, Vietnam."}]}},{"fullName":"Moss AF","firstName":"Amy F","lastName":"Moss","initials":"AF","authorId":{"type":"ORCID","value":"0000-0002-8647-8448"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Environmental and Rural Science, Faculty of Science, Agriculture, Business and Law, University of New England, Armidale, NSW 2351, Australia."},{"affiliation":"Poultry Hub Australia, University of New England, Armidale, NSW 2350, Australia."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0002-3093-1207"},{"type":"ORCID","value":"0000-0002-8647-8448"}]},"journalInfo":{"issue":"24","volume":"15","journalIssueId":4079572,"dateOfPublication":"2025 Dec","monthOfPublication":12,"yearOfPublication":2025,"printPublicationDate":"2025-12-01","journal":{"title":"Animals : an open access journal from MDPI","medlineAbbreviation":"Animals (Basel)","isoabbreviation":"Animals (Basel)","nlmid":"101635614","essn":"2076-2615","issn":"2076-2615"}},"pubYear":"2025","pageInfo":"3609","abstractText":"Soybean meal has traditionally dominated poultry diets as the protein source. However, its widespread use raises concerns regarding economic costs, environmental impact and social sustainability. As a result, there is growing interest in alternative protein sources, such as canola meal, which may reduce feed costs while sustaining productivity. This review evaluates the potential of canola meal as a sustainable protein source in modern poultry production systems, focusing on nutritional, economic and environmental advantages, as well as the potential implications of canola meal inclusion in reduced-protein diets. Evidence from scientific studies indicates that canola meal's nutritional profile supports bird growth and production, although higher fiber content and anti-nutritional compounds reduce metabolizable energy, making it more suitable for laying hens than broiler chickens. Processing techniques, enzyme supplementation, fermentation, and modern cultivars have improved both nutritional value and practical utility of canola meal. Performance outcomes differ by species. Broilers exhibit variable growth at high inclusion levels, whereas laying hens are estimated to tolerate up to 20% without affecting laying performance or egg quality; however, data is severely lacking, particularly under the context of modern reduced-protein diets. Economically, canola meal is cost-competitive with soybean meal. From an environmental perspective, substituting imported soybean meal with local canola reduces greenhouse gas emissions, enhances resource efficiency, and supports pollinators. Nevertheless, trade-offs exist, including increased land use, variable digestibility, and potential eutrophication. Incorporating canola meal into reduced-protein diets offers both economic and ecological benefits, though effectiveness depends on the extent of protein reduction and the precision of amino acid formulation. Overall, canola meal offers a sustainable, economically viable, and environmentally responsible protein source for modern poultry production, provided that inclusion levels are adjusted to species-specific requirements and regional conditions.","affiliation":"School of Environmental and Rural Science, Faculty of Science, Agriculture, Business and Law, University of New England, Armidale, NSW 2351, Australia.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"grantsList":{"grant":[{"grantId":"INNO020","agency":"Australian Eggs Limited","orderIn":0}]},"keywordList":{"keyword":["Environment","Sustainability","Nutrition","Layer hen","rapeseed","Broiler Chicken","Economic","Canola Meal"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/ani15243609"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12729943"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12729943?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2025-12-30","dateOfCreation":"2025-12-30","firstIndexDate":"2026-01-02","fullTextReceivedDate":"2025-12-26","dateOfRevision":"2026-01-02","electronicPublicationDate":"2025-12-15","firstPublicationDate":"2025-12-15"},{"id":"42591868","source":"MED","pmid":"42591868","doi":"10.3389/fpls.2026.1874927","title":"Integrated multi-omics analysis reveals candidate genes for cuticular wax biosynthesis and molecular characteristics of a glossy mutant in rapeseed under natural drought stress.","authorString":"Lei L, Meng X, Wang W, Li H, Zhu J.","authorList":{"author":[{"fullName":"Lei L","firstName":"Lei","lastName":"Lei","initials":"L","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China."}]}},{"fullName":"Meng X","firstName":"Xianmin","lastName":"Meng","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China."}]}},{"fullName":"Wang W","firstName":"Weirong","lastName":"Wang","initials":"W","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China."}]}},{"fullName":"Li H","firstName":"Hongwei","lastName":"Li","initials":"H","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China."}]}},{"fullName":"Zhu J","firstName":"Jifeng","lastName":"Zhu","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China."}]}}]},"journalInfo":{"volume":"17","journalIssueId":4112080,"dateOfPublication":"2026 ","monthOfPublication":0,"yearOfPublication":2026,"printPublicationDate":"2026-01-01","journal":{"title":"Frontiers in plant science","medlineAbbreviation":"Front Plant Sci","isoabbreviation":"Front Plant Sci","nlmid":"101568200","essn":"1664-462X","issn":"1664-462X"}},"pubYear":"2026","pageInfo":"1874927","abstractText":"<h4>Introduction</h4>Cuticular wax plays a crucial role in drought tolerance. However, the regulatory mechanisms controlling cuticular wax biosynthesis and the drought response in rapeseed are not well understood.<h4>Methods and results</h4>In this study, we identified a glossy mutant, <i>hy7201</i>, in rapeseed (<i>Brassica napus</i> L.). Compared to the wild-type 'HY7201', <i>hy7201</i> exhibited a significant reduction in total cuticular wax content, altered composition, and decreased crystal density, along with a significant increase in cuticle permeability. Genetic analysis revealed that the glossy phenotype of the <i>hy7201</i> mutant is controlled by a single dominant gene. Through bulked segregant analysis coupled with next-generation sequencing (BSA-seq) of waxy and glossy pools derived from F1 individuals of the self-crossed <i>hy7201</i> population, <i>BnaA09G0721400ZS</i>, the homolog gene of <i>AtCER1</i>, was identified as a key candidate gene. This gene encodes a very-long-chain aldehyde decarbonylase, which contributes to the differences in leaf cuticular wax accumulation between the wild-type 'HY7201' and the glossy mutant '<i>hy7201</i>'. To validate the key genes involved in cuticular wax biosynthesis and characterize the molecular features under natural drought conditions in glossy plants, we performed an integrated analysis of the leaf transcriptome, proteome, and metabolome using KEGG enrichment, Pearson correlation, and two-way orthogonal partial least squares (O2PLS) methods with three biological replicates. The genes CER1 (<i>BnaA09G0721400ZS</i>) and its paralog <i>CER1-2</i> (<i>BnaA09G0698500ZS</i>) were significantly downregulated at both the transcriptional and protein levels. Additionally, they exhibited significant negative correlations with differentially expressed metabolites (DEMs) in glossy plants under drought stress. The multi-omics approach uncovered that pathways related to cutin, suberin, and wax biosynthesis, as well as ABC transporters, glucosinolate biosynthesis, glutathione metabolism, linoleic acid metabolism, sphingolipid metabolism, and arginine and proline metabolism, significantly contribute to the glossy plant's response to drought.<h4>Discussion</h4>These results suggest that the drought response in the glossy mutant involves not only a defective cuticular barrier but also extensive metabolic and signaling reprogramming. This insight could facilitate the identification of genes related to cuticular wax biosynthesis and drought stress response, which could be utilized in molecular breeding programs to enhance drought tolerance in rapeseed. Consequently, this offers a promising approach for developing more sustainable farming methods amid environmental challenges.","affiliation":"Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic-eCollection","pubTypeList":{"pubType":["Journal Article"]},"keywordList":{"keyword":["Brassica napus L.","Drought","Cuticular Wax","Cer1","Multi-omics Analysis"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3389/fpls.2026.1874927"}]},"isOpenAccess":"N","inEPMC":"N","inPMC":"N","hasPDF":"N","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-08-13","dateOfCreation":"2026-08-13","firstIndexDate":"2026-08-15","dateOfRevision":"2026-08-14","electronicPublicationDate":"2026-07-29","firstPublicationDate":"2026-07-29"},{"id":"42497668","source":"MED","pmid":"42497668","doi":"10.1016/j.plaphy.2026.111558","title":"Priming-induced drought memory in rapeseed and cotton: A meta-analytic assessment.","authorString":"Hashemi A, Faghani E, Roitsch TG.","authorList":{"author":[{"fullName":"Hashemi A","firstName":"Amenehsadat","lastName":"Hashemi","initials":"A","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Agriculture, University of Applied Science and Technology, Tehran, Iran."}]}},{"fullName":"Faghani E","firstName":"Elham","lastName":"Faghani","initials":"E","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Agrnomy Cotton Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, Iran. Electronic address: elhamfaghanibio@gmail.com."}]}},{"fullName":"Roitsch TG","firstName":"Thomas Georg","lastName":"Roitsch","initials":"TG","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Environmental Sciences, Aarhus University, Roskilde, Denmark."}]}}]},"journalInfo":{"volume":"237","journalIssueId":4242040,"dateOfPublication":"2026 Aug","monthOfPublication":8,"yearOfPublication":2026,"printPublicationDate":"2026-08-01","journal":{"title":"Plant physiology and biochemistry : PPB","medlineAbbreviation":"Plant Physiol Biochem","isoabbreviation":"Plant Physiol Biochem","nlmid":"9882449","essn":"1873-2690","issn":"0981-9428"}},"pubYear":"2026","pageInfo":"111558","abstractText":"Seed priming is increasingly used to enhance crop resilience to drought, yet whether it induces functional stress memory, and how this varies across crops, remains unclear. We conducted a global meta-analysis of 28 studies comprising 761 effect sizes to evaluate priming-induced drought memory in two major crops, rapeseed (Brassica napus) and cotton (Gossypium hirsutum). Effect sizes were calculated as standardized mean differences (Hedges' g) and synthesized using a random-effects model. Responses were evaluated across physiological, biochemical, and yield-related traits, while drought intensity and priming type were tested as key moderators to explain variation among studies. Priming significantly improved drought tolerance in both crops, but through divergent strategies: rapeseed responses were generally consistent with enhanced cellular protection via robust antioxidant activation (superoxide dismutase, catalase, ascorbate peroxidase) and reduced oxidative damage (malondialdehyde and hydrogen peroxide), particularly under moderate drought stress. In contrast, cotton may reflect a reproductive assurance strategy, sustaining leaf water status (RWC), accumulating proline, and significantly enhancing seed and boll yield, even when antioxidant responses were non-significant. Nutrient/mineral and microbial priming were most effective in cotton, whereas nutrient/mineral and hormonal/biochemical priming excelled in rapeseed. High heterogeneity in cotton (I<sup>2</sup> = 90.99%) indicates that priming responses are strongly influenced by experimental context, including drought severity and priming treatment. Overall, our findings are compatible with a functional, phenotypic interpretation of stress-memory-related responses rather than direct evidence of epigenetic memory, and highlight the potential value of crop-tailored priming strategies for improving drought resilience and supporting climate-smart agricultural production under increasingly water-limited conditions.","affiliation":"Department of Agriculture, University of Applied Science and Technology, Tehran, Iran.","publicationStatus":"ppublish","language":"eng","pubModel":"Print-Electronic","pubTypeList":{"pubType":["Meta-Analysis","Journal Article"]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"ME","qualifierName":"metabolism","majorTopic_YN":"N"},{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Brassica rapa","meshQualifierList":{"meshQualifier":[{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Gossypium","meshQualifierList":{"meshQualifier":[{"abbreviation":"ME","qualifierName":"metabolism","majorTopic_YN":"N"},{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Seeds","meshQualifierList":{"meshQualifier":[{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Stress, Physiological"},{"majorTopic_YN":"Y","descriptorName":"Droughts"},{"majorTopic_YN":"N","descriptorName":"Drought Resistance"}]},"keywordList":{"keyword":["Drought stress","Meta-analysis","Seed priming","Stress Memory","Crop Resilience"]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1016/j.plaphy.2026.111558"}]},"isOpenAccess":"N","inEPMC":"N","inPMC":"N","hasPDF":"N","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by-nc-nd","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-08-11","dateOfCreation":"2026-07-24","firstIndexDate":"2026-07-26","dateOfRevision":"2026-08-11","electronicPublicationDate":"2026-07-21","firstPublicationDate":"2026-07-21"},{"id":"41775912","source":"MED","pmid":"41775912","doi":"10.1007/s00122-026-05191-z","title":"Natural allelic variation in BnCLV1 orchestrates root architectural remodelling and yield performance under nitrogen-limited conditions in Brassica napu.","authorString":"Wang X, Du Y, Cui B, Xu C, Niu J, Wang X, Xu P.","authorList":{"author":[{"fullName":"Wang X","firstName":"Xiaohua","lastName":"Wang","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agriculture and Forestry Science, Linyi University, Middle of Shuangling Road, Lanshan District, Linyi, 276000, China."}]}},{"fullName":"Du Y","firstName":"Yi","lastName":"Du","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"State Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, China."}]}},{"fullName":"Cui B","firstName":"Baixi","lastName":"Cui","initials":"B","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agriculture and Forestry Science, Linyi University, Middle of Shuangling Road, Lanshan District, Linyi, 276000, China."}]}},{"fullName":"Xu C","firstName":"Chuanquan","lastName":"Xu","initials":"C","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agriculture and Forestry Science, Linyi University, Middle of Shuangling Road, Lanshan District, Linyi, 276000, China."}]}},{"fullName":"Niu J","firstName":"Jie","lastName":"Niu","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agriculture and Forestry Science, Linyi University, Middle of Shuangling Road, Lanshan District, Linyi, 276000, China."}]}},{"fullName":"Wang X","firstName":"Xiaohong","lastName":"Wang","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Shandong Provincial Forestry Protection and Development Service Center, Ji'nan, 250014, China."}]}},{"fullName":"Xu P","firstName":"Ping","lastName":"Xu","initials":"P","authorId":{"type":"ORCID","value":"0000-0001-6099-1748"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agriculture and Forestry Science, Linyi University, Middle of Shuangling Road, Lanshan District, Linyi, 276000, China. miao9921@126.com."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-6099-1748"}]},"journalInfo":{"issue":"3","volume":"139","journalIssueId":4139781,"dateOfPublication":"2026 Mar","monthOfPublication":3,"yearOfPublication":2026,"printPublicationDate":"2026-03-01","journal":{"title":"TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik","medlineAbbreviation":"Theor Appl Genet","isoabbreviation":"Theor Appl Genet","nlmid":"0145600","essn":"1432-2242","issn":"1432-2242"}},"pubYear":"2026","pageInfo":"83","abstractText":"<h4>Key message</h4>The findings reveal that BnCLV1 modulates root growth and yield under N-deficient conditions in rapeseed, which may offering a target for breeding varieties with enhanced N use efficiency and improved agricultural sustainability. Nitrogen (N) deficiency is a major limiting factor for rapeseed root growth and yield. Therefore, genetic improvement of rapeseed with N acquisition and utilisation efficiency is a curial strategy for enhancing rapeseed yield and N fertiliser efficiency. Using genome-wide association study (GWAS) and transcriptome analyses, we found that natural variation of leucine-rich repeat receptor-like kinase gene (BnCLV1) regulates N-responsive root system architecture at the seeding stage and yield at the maturity in the natural rapeseed population. N supply inhibited BnCLV1 expression, thereby increasing lateral root number, root surface area, shoot and root biomass, and total root length at the seeding stage. N supply also enhanced yield, pod number and silique length in the maturation stage. GUS assays showed that BnCLV1 signals were negatively regulated in response to N deficiency in primary root, lateral root primordia, leaf veins, and floral primordia tissues. Thus, BnCLV1 modulates lateral root and floral primordia activity, reducing root growth and yield under N deficiency. Overall, these findings reveal that BnCLV1 modulates root growth and yield under N deficiency in rapeseed, providing a target for breeding varieties with enhanced N-use efficiency and improved agricultural sustainability.","affiliation":"College of Agriculture and Forestry Science, Linyi University, Middle of Shuangling Road, Lanshan District, Linyi, 276000, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["Journal Article"]},"grantsList":{"grant":[{"grantId":"32201868","agency":"National Natural Science Foundation of China","orderIn":0},{"grantId":"ZR2024MC152","agency":"Natural Science Foundation of Shandong Province","orderIn":0}]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"},{"abbreviation":"GD","qualifierName":"growth & 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Single Nucleotide"},{"majorTopic_YN":"N","descriptorName":"Alleles"},{"majorTopic_YN":"N","descriptorName":"Genetic Variation"},{"majorTopic_YN":"N","descriptorName":"Genome-Wide Association Study"}]},"chemicalList":{"chemical":[{"name":"Plant Proteins","registryNumber":"0"},{"name":"Nitrogen","registryNumber":"N762921K75"}]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1007/s00122-026-05191-z"}]},"isOpenAccess":"N","inEPMC":"N","inPMC":"N","hasPDF":"N","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-07-10","dateOfCreation":"2026-03-03","firstIndexDate":"2026-03-04","dateOfRevision":"2026-07-10","electronicPublicationDate":"2026-03-03","firstPublicationDate":"2026-03-03"},{"id":"42137213","source":"MED","pmid":"42137213","pmcid":"PMC13167431","fullTextIdList":{"fullTextId":["PMC13167431"]},"doi":"10.3389/fpls.2026.1813997","title":"Lipid regulation in &lt;i&gt;Brassica napus&lt;/i&gt;: spatiotemporal studies to enhance our understanding.","authorString":"Liao P, Lechon T, Harwood JL, Scofield S.","authorList":{"author":[{"fullName":"Liao P","firstName":"Pan","lastName":"Liao","initials":"P","authorId":{"type":"ORCID","value":"0000-0002-1156-8916"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China."},{"affiliation":"State Key Laboratory of Agrobiotechnology (CUHK), Shatin, Hong Kong SAR, China."}]}},{"fullName":"Lechon T","firstName":"Tamara","lastName":"Lechon","initials":"T","authorId":{"type":"ORCID","value":"0000-0002-5163-5132"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Biosciences, Cardiff University, Cardiff, United Kingdom."}]}},{"fullName":"Harwood JL","firstName":"John L","lastName":"Harwood","initials":"JL","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Biosciences, Cardiff University, Cardiff, United Kingdom."}]}},{"fullName":"Scofield S","firstName":"Simon","lastName":"Scofield","initials":"S","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Biosciences, Cardiff University, Cardiff, United Kingdom."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0002-1156-8916"},{"type":"ORCID","value":"0000-0002-5163-5132"}]},"dataLinksTagsList":{"dataLinkstag":["altmetrics","supporting_data"]},"journalInfo":{"volume":"17","journalIssueId":4112080,"dateOfPublication":"2026 ","monthOfPublication":0,"yearOfPublication":2026,"printPublicationDate":"2026-01-01","journal":{"title":"Frontiers in plant science","medlineAbbreviation":"Front Plant Sci","isoabbreviation":"Front Plant Sci","nlmid":"101568200","essn":"1664-462X","issn":"1664-462X"}},"pubYear":"2026","pageInfo":"1813997","abstractText":"<i>Brassica napus</i> (and related <i>Brassica</i> species) is the world's third most important oil crop, providing around 13% of the total global vegetable oil. There are two basic types of cultivars - those with significant erucate being used for renewable chemicals, while low-erucate varieties (the majority) supply the food industry. <i>B. napus</i> oil is particularly enriched in oleate but also contains a nutritionally desirable ratio of <i>n</i>-3 to <i>n</i>-6 polyunsaturated fatty acids. In this review we note the overall importance of rapeseed oil and describe in detail how it is biosynthesised, mainly through the classic Kennedy pathway with additional reactions. We then discuss very recent advances in our understanding of how biosynthesis is regulated and spatiotemporal aspects of oil accumulation in the crop. Both biotic and abiotic environmental effects on <i>B.napus</i> yields are then summarized. Recently, MALDI-MSI has been developed for lipids and its ability to reveal spatial and temporal differences in lipid species distribution has proven especially useful. The technique has exposed unsuspected details in metabolism as well as confirming other reported aspects of lipid biochemistry. The similar, but not identical, lipid metabolism in Arabidopsis has facilitated many of the advances in <i>B. napus</i> and it is anticipated that the momentum of new discoveries will continue to be rapid and significant.","affiliation":"Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic-eCollection","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"keywordList":{"keyword":["Fatty acid","Brassica napus","Oilseed rape","Canola","Lipid Regulation","Maldi- Ms"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3389/fpls.2026.1813997"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13167431"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13167431?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"Y","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-05-15","dateOfCreation":"2026-05-15","firstIndexDate":"2026-05-16","fullTextReceivedDate":"2026-05-20","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-04-29","firstPublicationDate":"2026-04-29"},{"id":"41191609","source":"MED","pmid":"41191609","pmcid":"PMC12588495","fullTextIdList":{"fullTextId":["PMC12588495"]},"doi":"10.1371/journal.pone.0335220","title":"Effect of wheat straw biochar addition on canola growth in different soils.","authorString":"Hassan M, Strezov V.","authorList":{"author":[{"fullName":"Hassan M","firstName":"Masooma","lastName":"Hassan","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Natural Sciences, Faculty of Science and Engineering, Macquarie University NSW, Sydney, New South Wales, Australia."}]}},{"fullName":"Strezov V","firstName":"Vladimir","lastName":"Strezov","initials":"V","authorId":{"type":"ORCID","value":"0000-0002-9129-9284"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Natural Sciences, Faculty of Science and Engineering, Macquarie University NSW, Sydney, New South Wales, Australia."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0002-9129-9284"}]},"dataLinksTagsList":{"dataLinkstag":["related_data"]},"journalInfo":{"issue":"11","volume":"20","journalIssueId":4041491,"dateOfPublication":"2025 ","monthOfPublication":0,"yearOfPublication":2025,"printPublicationDate":"2025-01-01","journal":{"title":"PloS one","medlineAbbreviation":"PLoS One","isoabbreviation":"PLoS One","nlmid":"101285081","essn":"1932-6203","issn":"1932-6203"}},"pubYear":"2025","pageInfo":"e0335220","abstractText":"Biochar has been demonstrated as a soil amendment to improve soil health and plant yield. The present study aimed at investigating the potential of wheat straw biochar on canola morphology and yield grown in different soils. The influence of biochar on soil physical and chemical properties was also assessed. A completely randomised design pot experiment was carried out in glasshouse where canola was planted in eight different soils with and without biochar treatment. Wheat straw biochar was incorporated in pots at 1% of the total soil weight. Canola was grown for 105 days after which its morphological and yield parameters were evaluated. Analysis of variance confirmed that biochar exerted a significant effect on shoot length, shoot and root dry weights, flower count and 100 seeds weight. Soil texture also affected canola growth and yield parameters with higher clay content in clay loam resulting in less yield compared to others. Biochar also led to improved leaf fresh and dry weight, shoot and root dry weight in loam with lower seeds weight. The seeds weight was the greatest in sandy clay loam, silty clay and silty clay loam which could be ascribed to pH changes, soil texture, decline in soil particle density and improved nutrient availability. Biochar also inflluenced increase in carbon, nitrogen and potassium levels which all helped in maximizing the yield.","affiliation":"School of Natural Sciences, Faculty of Science and Engineering, Macquarie University NSW, Sydney, New South Wales, Australia.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic-eCollection","pubTypeList":{"pubType":["research-article","Journal Article"]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Triticum","meshQualifierList":{"meshQualifier":[{"abbreviation":"CH","qualifierName":"chemistry","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Seeds","meshQualifierList":{"meshQualifier":[{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Plant Roots","meshQualifierList":{"meshQualifier":[{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Charcoal","meshQualifierList":{"meshQualifier":[{"abbreviation":"PD","qualifierName":"pharmacology","majorTopic_YN":"N"},{"abbreviation":"CH","qualifierName":"chemistry","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Nitrogen","meshQualifierList":{"meshQualifier":[{"abbreviation":"AN","qualifierName":"analysis","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Soil","meshQualifierList":{"meshQualifier":[{"abbreviation":"CH","qualifierName":"chemistry","majorTopic_YN":"N"}]}}]},"chemicalList":{"chemical":[{"name":"Charcoal","registryNumber":"16291-96-6"},{"name":"Soil","registryNumber":"0"},{"name":"biochar","registryNumber":"0"},{"name":"Nitrogen","registryNumber":"N762921K75"}]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1371/journal.pone.0335220"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12588495"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12588495?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"Y","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"Y","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2025-11-05","dateOfCreation":"2025-11-05","firstIndexDate":"2025-11-05","fullTextReceivedDate":"2025-11-08","dateOfRevision":"2025-11-08","electronicPublicationDate":"2025-11-05","firstPublicationDate":"2025-11-05"},{"id":"40647951","source":"MED","pmid":"40647951","pmcid":"PMC12252070","fullTextIdList":{"fullTextId":["PMC12252070"]},"doi":"10.3390/plants14131942","title":"Impact of Water Deficit Stress on &lt;i&gt;Brassica&lt;/i&gt; Crops: Growth and Yield, Physiological and Biochemical Responses.","authorString":"Mohan VR, MacDonald MT, Abbey L.","authorList":{"author":[{"fullName":"Mohan VR","firstName":"Vijaya R","lastName":"Mohan","initials":"VR","authorId":{"type":"ORCID","value":"0009-0002-8142-7884"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Bible Hill, NS B2N 5E3, Canada."}]}},{"fullName":"MacDonald MT","firstName":"Mason T","lastName":"MacDonald","initials":"MT","authorId":{"type":"ORCID","value":"0000-0003-0818-0141"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Bible Hill, NS B2N 5E3, Canada."}]}},{"fullName":"Abbey L","firstName":"Lord","lastName":"Abbey","initials":"L","authorId":{"type":"ORCID","value":"0000-0003-2219-1752"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Bible Hill, NS B2N 5E3, Canada."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0003-0818-0141"},{"type":"ORCID","value":"0000-0003-2219-1752"},{"type":"ORCID","value":"0009-0002-8142-7884"}]},"journalInfo":{"issue":"13","volume":"14","journalIssueId":3977027,"dateOfPublication":"2025 Jun","monthOfPublication":6,"yearOfPublication":2025,"printPublicationDate":"2025-06-01","journal":{"title":"Plants (Basel, Switzerland)","medlineAbbreviation":"Plants (Basel)","isoabbreviation":"Plants (Basel)","nlmid":"101596181","essn":"2223-7747","issn":"2223-7747"}},"pubYear":"2025","pageInfo":"1942","abstractText":"Drought including both meteorological drought and water deficiency stress conditions is a major constraint on global agricultural productivity, particularly affecting <i>Brassica</i> species, which are vital oilseed and vegetable crops. As climate change intensifies, understanding plant responses to drought is crucial for improving drought resilience. Drought stress impacts <i>Brassica</i> crops at multiple levels, reducing germination rates, impairing physiological functions such as photosynthesis and water-use efficiency, and triggering oxidative stress due to the accumulation of reactive oxygen species. To counteract these effects, <i>Brassica</i> plants employ various adaptive mechanisms, including osmotic adjustment, antioxidant defense activation, and hormonal regulation. Recent research has explored molecular and physiological pathways involved in drought tolerance, revealing key physiological changes and biochemical markers that could be targeted for crop improvement. This review summarizes the latest findings on the physiological, biochemical, and molecular responses of <i>Brassica</i> crops to drought stress, with an emphasis on adaptive mechanisms and potential drought mitigation strategies. Additionally, future research directions are proposed, focusing on integrating molecular and agronomic approaches to enhance drought resilience in <i>Brassica</i> species.","affiliation":"Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Bible Hill, NS B2N 5E3, Canada.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"keywordList":{"keyword":["Brassica","Photosynthesis","Tolerance","Climate change","Water deficit stress","Drought","Mechanisms"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/plants14131942"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12252070"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12252070?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":6,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCreation":"2025-07-12","firstIndexDate":"2025-07-14","fullTextReceivedDate":"2025-11-29","dateOfRevision":"2025-07-14","electronicPublicationDate":"2025-06-24","firstPublicationDate":"2025-06-24"},{"id":"41683082","source":"MED","pmid":"41683082","pmcid":"PMC12896430","fullTextIdList":{"fullTextId":["PMC12896430"]},"doi":"10.3390/foods15030498","title":"Rapid Protein Extraction from Canola Meal Pre-Treated with Enzymatic Reactive Extrusion.","authorString":"Ghosh S, González Hernández EC, Sha X, Chow J, San Martin-Gonzalez F, Jin Q, Chen D.","authorList":{"author":[{"fullName":"Ghosh S","firstName":"Sunandita","lastName":"Ghosh","initials":"S","authorId":{"type":"ORCID","value":"0000-0002-7704-0858"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA."}]}},{"fullName":"González Hernández EC","firstName":"Edith Cristina","lastName":"González Hernández","initials":"EC","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA."}]}},{"fullName":"Sha X","firstName":"Xinmei","lastName":"Sha","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA."}]}},{"fullName":"Chow J","firstName":"Jeff","lastName":"Chow","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA."}]}},{"fullName":"San Martin-Gonzalez F","firstName":"Fernanda","lastName":"San Martin-Gonzalez","initials":"F","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA."}]}},{"fullName":"Jin Q","firstName":"Qing","lastName":"Jin","initials":"Q","authorId":{"type":"ORCID","value":"0000-0002-2377-5218"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Food and Agriculture, University of Maine, Orono, ME 04469, USA."}]}},{"fullName":"Chen D","firstName":"Da","lastName":"Chen","initials":"D","authorId":{"type":"ORCID","value":"0000-0003-3830-0730"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0002-2377-5218"},{"type":"ORCID","value":"0000-0002-7704-0858"},{"type":"ORCID","value":"0000-0003-3830-0730"}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"3","volume":"15","journalIssueId":4120565,"dateOfPublication":"2026 Feb","monthOfPublication":2,"yearOfPublication":2026,"printPublicationDate":"2026-02-01","journal":{"title":"Foods (Basel, Switzerland)","medlineAbbreviation":"Foods","isoabbreviation":"Foods","nlmid":"101670569","essn":"2304-8158","issn":"2304-8158"}},"pubYear":"2026","pageInfo":"498","abstractText":"Conventional alkaline extraction of plant proteins typically requires highly alkaline conditions (pH ≥ 11) and extended extraction times (~1 h). Although protease addition can lower extraction pH and improve functionality, it often requires prolonged hydrolysis. In this study, enzymatic reactive extrusion (<i>eREX</i>) using Alcalase, followed by a short duration alkaline extraction (5 min, pH 9), was evaluated as an alternative approach for producing protein-rich extracts from canola meal. The <i>eREX</i> process increased protein recovery by 48% and 42% compared with alkaline extraction conducted without and with Alcalase, respectively. The resulting powdered extracts reached a protein content of up to 49% and consisted primarily of partially hydrolyzed proteins (10-23 kDa) with increased surface hydrophobicity. Amino acid analysis showed substantial enrichment of essential amino acids, particularly histidine and sulfur-containing amino acids. Functional properties were improved, including enhanced solubility across pH 2-10, high foaming stability (88%), and increased oil-binding capacity (~5.5 g g<sup>-1</sup>), while in vitro digestibility remained comparable (~85%). Techno-economic analysis indicated reductions in water use (~11%), energy consumption (~48%), and production cost (16-25%). Overall, <i>eREX</i> provides a rapid, higher-throughput, and cost-effective strategy for producing premium canola protein ingredients.","affiliation":"Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"grantsList":{"grant":[{"grantId":"24-PB-US-FC-1-867","agency":"Good Food Institute","orderIn":0}]},"keywordList":{"keyword":["Plant proteins","Amino acid composition","Limited hydrolysis","In vitro digestion","Functionality","Thermal Mechanical Treatment"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/foods15030498"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12896430"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12896430?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"Y","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"Y","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-02-13","dateOfCreation":"2026-02-13","firstIndexDate":"2026-02-13","fullTextReceivedDate":"2026-02-14","dateOfRevision":"2026-02-16","electronicPublicationDate":"2026-02-01","firstPublicationDate":"2026-02-01"},{"id":"42197461","source":"MED","pmid":"42197461","pmcid":"PMC13210192","fullTextIdList":{"fullTextId":["PMC13210192"]},"doi":"10.3390/microorganisms14051075","title":"Soil Microbial Dynamics in Regenerative Agriculture Systems: A Data-Driven Synthesis for Soil Health, Pest Suppression, and Yield Sustainability in the Western Canadian Prairies.","authorString":"Nishu SD, Islam MN.","authorList":{"author":[{"fullName":"Nishu SD","firstName":"Susmita Das","lastName":"Nishu","initials":"SD","authorId":{"type":"ORCID","value":"0009-0007-5191-7045"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Environment and Sustainability, University of Saskatchewan, Saskatoon, SK S7N 5A2, Canada."}]}},{"fullName":"Islam MN","firstName":"M Nazrul","lastName":"Islam","initials":"MN","authorId":{"type":"ORCID","value":"0000-0001-6388-397X"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Graduate and Postdoctoral Studies, University of Saskatchewan, Saskatoon, SK S7N 5A2, Canada."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-6388-397X"},{"type":"ORCID","value":"0009-0007-5191-7045"}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"5","volume":"14","journalIssueId":4201735,"dateOfPublication":"2026 May","monthOfPublication":5,"yearOfPublication":2026,"printPublicationDate":"2026-05-01","journal":{"title":"Microorganisms","medlineAbbreviation":"Microorganisms","isoabbreviation":"Microorganisms","nlmid":"101625893","essn":"2076-2607","issn":"2076-2607"}},"pubYear":"2026","pageInfo":"1075","abstractText":"Regenerative agriculture (RA) is expanding across the Western Canadian Prairies, but its microbial foundations under climatic constraint remain insufficiently integrated. This review synthesizes evidence from long-term Prairie field experiments, regional and global datasets to evaluate how regenerative management reshapes soil biological processes and agronomic performance across systems. RA practices including no-till, diversified rotations, cover cropping, and organic amendments consistently enhance microbial biomass (up to 40-86%), arbuscular mycorrhizal fungal abundance (32-60%), and microbial diversity (≈50%), alongside increases in soil organic carbon (up to 15.6 kg C ha<sup>-1</sup> yr<sup>-1</sup>), aggregate stability (up to 38%), and water retention (up to 30-34%). These biologically mediated improvements are linked to enhanced nutrient cycling and crop nitrogen uptake (13-47%), as well as increased microbial enzymatic activity and functional gene abundance. Agronomically, these changes translate into yield gains ranging from 10% to 147% under long-term no-till and 14-38% under diversified rotations, with additional system-level benefits including reductions in synthetic nitrogen inputs (up to 73%) and herbicide use (up to 42%). While agronomic benefits vary across temporal scales and environmental conditions, this synthesis identifies microbial communities as key mediators of interactions among climate, plant, and soil systems, underpinning improvements in soil health, pest suppression, and yield stability in semi-arid, climate-variable Prairie agroecosystems. Continued long-term, system-level research is needed to refine regionally adapted regenerative transitions and to clarify how microbial processes mediate resilience under future climate uncertainty.","affiliation":"Department of Environment and Sustainability, University of Saskatchewan, Saskatoon, SK S7N 5A2, Canada.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"keywordList":{"keyword":["soil health","Pest Suppression","Cover Cropping","Reduced Tillage","Regenerative Agriculture","Soil Microbial Dynamics","Western Canadian Prairies","Yield Sustainability","Diversified Rotation"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/microorganisms14051075"},{"availability":"Open 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by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-05-27","dateOfCreation":"2026-05-27","firstIndexDate":"2026-05-28","fullTextReceivedDate":"2026-05-27","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-05-09","firstPublicationDate":"2026-05-09"},{"id":"41829816","source":"MED","pmid":"41829816","pmcid":"PMC12987265","fullTextIdList":{"fullTextId":["PMC12987265"]},"doi":"10.3390/plants15050787","title":"Early Plant Development as a Systems-Level Trait: Integrating Omics, Artificial Intelligence, and Emerging Biotechnologies.","authorString":"Al-Sawa'eer AS, Al-Samydai A, Odeh L, Haj Ahmad F, Obekh R, Elqader YMA, Khaleel A, Al-Athamneh AM, Gabriele M, Di Simone SC, Ferrante C, Menghini L, Ali Agha ASA.","authorList":{"author":[{"fullName":"Al-Sawa'eer AS","firstName":"Abdallah S","lastName":"Al-Sawa'eer","initials":"AS","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"National Seeds Production Company, Amman 11183, Jordan."}]}},{"fullName":"Al-Samydai A","firstName":"Ali","lastName":"Al-Samydai","initials":"A","authorId":{"type":"ORCID","value":"0000-0003-0093-2310"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Pharmaceutics and Pharmaceutical Technology, Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19111, Jordan."}]}},{"fullName":"Odeh L","firstName":"Lama","lastName":"Odeh","initials":"L","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biology and Biotechnology, Faculty of Science, The Hashemite University, Zarqa 13133, Jordan."}]}},{"fullName":"Haj Ahmad F","firstName":"Fatima","lastName":"Haj Ahmad","initials":"F","authorId":{"type":"ORCID","value":"0000-0001-5347-3918"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt 19117, Jordan."}]}},{"fullName":"Obekh R","firstName":"Renata","lastName":"Obekh","initials":"R","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biology and Biotechnology, Faculty of Science, The Hashemite University, Zarqa 13133, Jordan."}]}},{"fullName":"Elqader YMA","firstName":"Yousef M Abd","lastName":"Elqader","initials":"YMA","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"National Seeds Production Company, Amman 11183, Jordan."}]}},{"fullName":"Khaleel A","firstName":"Anas","lastName":"Khaleel","initials":"A","authorId":{"type":"ORCID","value":"0000-0001-7584-2438"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, Jordan."}]}},{"fullName":"Al-Athamneh AM","firstName":"Ahmad M","lastName":"Al-Athamneh","initials":"AM","authorId":{"type":"ORCID","value":"0000-0002-1387-1329"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Nutrition, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, Jordan."}]}},{"fullName":"Gabriele M","firstName":"Mariachiara","lastName":"Gabriele","initials":"M","authorId":{"type":"ORCID","value":"0009-0005-0774-2562"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Botanical Garden \"Giardino dei Semplici\", Department of Pharmacy, \"G. d'Annunzio\" University \"Chieti-Pescara\", Via dei Vestini n. 31, 66100 Chieti, Italy."}]}},{"fullName":"Di Simone SC","firstName":"Simonetta Cristina","lastName":"Di Simone","initials":"SC","authorId":{"type":"ORCID","value":"0000-0001-5041-3848"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Botanical Garden \"Giardino dei Semplici\", Department of Pharmacy, \"G. d'Annunzio\" University \"Chieti-Pescara\", Via dei Vestini n. 31, 66100 Chieti, Italy."}]}},{"fullName":"Ferrante C","firstName":"Claudio","lastName":"Ferrante","initials":"C","authorId":{"type":"ORCID","value":"0000-0001-9431-9407"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Botanical Garden \"Giardino dei Semplici\", Department of Pharmacy, \"G. d'Annunzio\" University \"Chieti-Pescara\", Via dei Vestini n. 31, 66100 Chieti, Italy."}]}},{"fullName":"Menghini L","firstName":"Luigi","lastName":"Menghini","initials":"L","authorId":{"type":"ORCID","value":"0000-0002-7346-7395"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Botanical Garden \"Giardino dei Semplici\", Department of Pharmacy, \"G. d'Annunzio\" University \"Chieti-Pescara\", Via dei Vestini n. 31, 66100 Chieti, Italy."}]}},{"fullName":"Ali Agha ASA","firstName":"Ahmed S A","lastName":"Ali Agha","initials":"ASA","authorId":{"type":"ORCID","value":"0009-0000-8516-2313"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman 11942, Jordan."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-5041-3848"},{"type":"ORCID","value":"0000-0001-5347-3918"},{"type":"ORCID","value":"0000-0001-7584-2438"},{"type":"ORCID","value":"0000-0001-9431-9407"},{"type":"ORCID","value":"0000-0002-1387-1329"},{"type":"ORCID","value":"0000-0002-7346-7395"},{"type":"ORCID","value":"0000-0003-0093-2310"},{"type":"ORCID","value":"0009-0000-8516-2313"},{"type":"ORCID","value":"0009-0005-0774-2562"}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"5","volume":"15","journalIssueId":4149975,"dateOfPublication":"2026 Mar","monthOfPublication":3,"yearOfPublication":2026,"printPublicationDate":"2026-03-01","journal":{"title":"Plants (Basel, Switzerland)","medlineAbbreviation":"Plants (Basel)","isoabbreviation":"Plants (Basel)","nlmid":"101596181","essn":"2223-7747","issn":"2223-7747"}},"pubYear":"2026","pageInfo":"787","abstractText":"Seed germination and early seedling development are critical determinants of crop establishment, stress tolerance, and yield stability, yet these stages remain insufficiently integrated into contemporary crop improvement strategies. Recent advances across genome editing, microbiome-assisted seed treatments, nanotechnology-enabled priming, and artificial intelligence-guided phenotyping have generated substantial but fragmented insights into early developmental regulation. This review synthesizes recent advances across early plant development research. It demonstrates that seemingly diverse technologies converge on a limited set of regulatory control nodes, including abscisic acid-gibberellin balance, redox homeostasis, and root system architectural plasticity. By integrating evidence from molecular, microbial, physicochemical, and computational studies, early plant ontogeny is presented as a tunable regulatory state governed by quantitative thresholds rather than as a strictly predetermined genetic process. Advances in deep learning, reinforcement learning, and high-throughput phenotyping further enable the modeling and optimization of early developmental trajectories across genotype by environment contexts. Together, these insights establish early development as a programmable target for crop improvement and provide a mechanistic foundation for designing integrated interventions that enhance developmental uniformity, stress resilience, and yield stability across diverse agroecological systems.","affiliation":"National Seeds Production Company, Amman 11183, Jordan.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"keywordList":{"keyword":["Artificial intelligence","Seed germination","Crispr-cas","Multi-omics","Nanopriming","Early Seedling Vigor"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/plants15050787"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12987265"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12987265?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":1,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-03-14","dateOfCreation":"2026-03-14","firstIndexDate":"2026-03-15","fullTextReceivedDate":"2026-03-14","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-03-04","firstPublicationDate":"2026-03-04"},{"id":"42196099","source":"MED","pmid":"42196099","pmcid":"PMC13205427","fullTextIdList":{"fullTextId":["PMC13205427"]},"doi":"10.3390/gels12050413","title":"Hydrogels for Agricultural Applications: From Soil Amendment to Crop Enhancement.","authorString":"Wang L, Hu J, Wang L, Xue X, Guo P, Dong Y, Xiao F, Li C, Guo L.","authorList":{"author":[{"fullName":"Wang L","firstName":"Luohui","lastName":"Wang","initials":"L","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China."},{"affiliation":"College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."}]}},{"fullName":"Hu J","firstName":"Jihang","lastName":"Hu","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China."}]}},{"fullName":"Wang L","firstName":"Liyun","lastName":"Wang","initials":"L","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China."}]}},{"fullName":"Xue X","firstName":"Xiaobo","lastName":"Xue","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China."}]}},{"fullName":"Guo P","firstName":"Panrong","lastName":"Guo","initials":"P","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China."}]}},{"fullName":"Dong Y","firstName":"Youming","lastName":"Dong","initials":"Y","authorId":{"type":"ORCID","value":"0000-0002-6496-3178"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China."}]}},{"fullName":"Xiao F","firstName":"Fei","lastName":"Xiao","initials":"F","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Hunan Academy of Forestry, Changsha 410018, China."}]}},{"fullName":"Li C","firstName":"Cheng","lastName":"Li","initials":"C","authorId":{"type":"ORCID","value":"0000-0001-5830-155X"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China."}]}},{"fullName":"Guo L","firstName":"Limin","lastName":"Guo","initials":"L","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0001-5830-155X"},{"type":"ORCID","value":"0000-0002-6496-3178"}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"5","volume":"12","journalIssueId":4201791,"dateOfPublication":"2026 May","monthOfPublication":5,"yearOfPublication":2026,"printPublicationDate":"2026-05-01","journal":{"title":"Gels (Basel, Switzerland)","medlineAbbreviation":"Gels","isoabbreviation":"Gels","nlmid":"101696925","essn":"2310-2861","issn":"2310-2861"}},"pubYear":"2026","pageInfo":"413","abstractText":"Hydrogels (HGs), three-dimensional cross-linked hydrophilic polymer networks, have emerged as a promising class of functional materials for sustainable agriculture due to their exceptional water retention capacity, responsiveness to environmental stimuli, and favorable biocompatibility. This review systematically summarizes the key functional properties of hydrogels and critically examines their multidimensional roles within agricultural systems. The major synergistic benefits of hydrogels are highlighted, including (1) improvement of soil physical structure, chemical properties, and the biological microenvironment, thereby facilitating soil remediation; (2) direct enhancement of seed germination, root development, and crop productivity when employed as soil amendments or seed-coating materials; (3) controlled and sustained release of water, nutrients (N, P, K, and trace elements), and pesticides, leading to significant improvements in resource use efficiency; (4) functional delivery of beneficial microorganisms, enabling precise regulation of their activity and efficacy; and (5) advancement of soilless cultivation technologies through the development of sophisticated hydrogel-based substrates. Furthermore, this review discusses the key challenges that currently limit large-scale agricultural implementation, including insufficient biodegradability, potential ecotoxicological risks, and techno-economic constraints. Finally, future research directions are proposed from an interdisciplinary perspective, emphasizing rational material design, performance optimization, and practical field application. This comprehensive review aims to provide systematic theoretical guidance and practical insights for the development and deployment of hydrogel-based technologies in sustainable agriculture.","affiliation":"College of Forestry, Henan Agricultural University, Zhengzhou 450002, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"grantsList":{"grant":[{"grantId":"CAFYBB2024MA033","agency":"the Fundamental Research Funds for the Central Non-profit Research Institution of CAF","orderIn":0},{"grantId":"CS2025A007","agency":"Quality and Safety Monitoring of Bamboo and Wood Products and Standard Formulation","orderIn":0},{"grantId":"30500928","agency":"Special Fund for Young Talents in Henan Agricultural University","orderIn":0},{"grantId":"CAFYBB2024MA033","agency":"Fundamental Research Funds for the Central Non-profit Research Institution of CAF","orderIn":0},{"grantId":"2025ZCY003","agency":"Development and industrialization of new technologies for processing and utilizing bamboo, as well as research and development of intelligent equipment","orderIn":0},{"grantId":"2025ZCY004","agency":"Standardization of bamboo and wood product quality and safety testing machines","orderIn":0},{"grantId":"30500928","agency":"the Special Fund for Young Talents in Henan Agricultural University","orderIn":0},{"grantId":"ZL2025A002","agency":"the Project for the Development of the Industry Category of Bamboo in the Context of Forest Conservation, Restoration, and Development in Hunan Province by 2025 (ZL2025A002): Devel-opment and industrialization of new technologies and intelligent equipment","orderIn":0}]},"keywordList":{"keyword":["Water retention","Crop growth","Sustainable agriculture","Soil amendment","hydrogel"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/gels12050413"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13205427"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13205427?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["doi"]},"dateOfCompletion":"2026-05-27","dateOfCreation":"2026-05-27","firstIndexDate":"2026-05-28","fullTextReceivedDate":"2026-05-27","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-05-09","firstPublicationDate":"2026-05-09"},{"id":"41491409","source":"MED","pmid":"41491409","pmcid":"PMC12870973","fullTextIdList":{"fullTextId":["PMC12870973"]},"doi":"10.1186/s12864-025-12496-8","title":"Genome-wide analysis of long noncoding RNAs in response to drought stress in the seeds of Brassica napus.","authorString":"Zhang K, Wang W, Li J, Liang Z, Li M.","authorList":{"author":[{"fullName":"Zhang K","firstName":"Kai","lastName":"Zhang","initials":"K","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China. kzhang@sxu.edu.cn."},{"affiliation":"Shanxi Baijiu Ecological Brewing Technology Innovation Center, Shanxi University, Taiyuan, Shanxi, 030006, China. kzhang@sxu.edu.cn."}]}},{"fullName":"Wang W","firstName":"Wenbing","lastName":"Wang","initials":"W","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China."},{"affiliation":"Shanxi Baijiu Ecological Brewing Technology Innovation Center, Shanxi University, Taiyuan, Shanxi, 030006, China."}]}},{"fullName":"Li J","firstName":"Junrong","lastName":"Li","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China."}]}},{"fullName":"Liang Z","firstName":"Zhen","lastName":"Liang","initials":"Z","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China."}]}},{"fullName":"Li M","firstName":"Maoteng","lastName":"Li","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Biological Seed Industry Research Institute, Xianghu Laboratory, Hangzhou, Zhejiang, 311231, China. limaoteng426@hust.edu.cn."},{"affiliation":"Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China. limaoteng426@hust.edu.cn."}]}}]},"journalInfo":{"issue":"1","volume":"27","journalIssueId":4082986,"dateOfPublication":"2026 Jan","monthOfPublication":1,"yearOfPublication":2026,"printPublicationDate":"2026-01-01","journal":{"title":"BMC genomics","medlineAbbreviation":"BMC Genomics","isoabbreviation":"BMC Genomics","nlmid":"100965258","essn":"1471-2164","issn":"1471-2164"}},"pubYear":"2026","pageInfo":"137","abstractText":"<h4>Background</h4>Long noncoding RNAs (lncRNAs) have been shown to play important roles in plant abiotic stress response and adaptation. However, the identification and characterization of genome-wide drought-responsive lncRNAs in rapeseed (Brassica napus L.) have been limited. Therefore, this study was the first to identify the expression profile of lncRNAs in rapeseed seeds responding to prolonged drought stress and subsequent short-term rewatering.<h4>Results</h4>A total of 6 000 lncRNAs were identified, among which 181 were classified as differentially expressed lncRNAs (DELs) in response to either drought stress or subsequent rewatering. Comparative analysis revealed that only 14 DELs were shared between the 159 DELs identified during drought stress and the 27 DELs detected upon rewatering. GO enrichment analysis showed that the co-expressed DEGs, primarily involved in photosynthesis, central carbon metabolism, stomatal movement, and strigolactone metabolism, were significantly down-regulated under drought stress but markedly up-regulated in subsequent rewatering. Furthermore, drought‑responsive competing endogenous RNA (ceRNA) networks were constructed based on the identified DETs and DEGs. Two ceRNA modules, MSTRG.57345.1-bna-miR164a/b/c/d-HSP2 and MSTRG.57345.1-bna-miR395d/e/f-HSFA7a, which are based on the newly identified lncRNA MSTRG.57345.1, were detected for the first time in rapeseed under drought stress and subsequent rewatering.<h4>Conclusions</h4>The present study advances our understanding of the expression patterns and functional role of rapeseed lncRNAs in the response to drought stress and the subsequent rewatering. It provides novel insights into lncRNA-mRNA networks and lncRNA-miRNA-mRNA networks in the seeds of B. napus. These findings offer a valuable reference for further genetic research and molecular breeding programs aimed at rapeseed improvement.","affiliation":"School of Life Science, Shanxi University, Taiyuan, Shanxi, 030006, China. kzhang@sxu.edu.cn.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"grantsList":{"grant":[{"grantId":"NO. 202403021222015","agency":"Shanxi Province Basic Research Program - Free Exploration Category","orderIn":0},{"grantId":"NO. 2024L006","agency":"Supported by Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi","orderIn":0},{"grantId":"NO. 32501951","agency":"National Natural Science Foundation of China","orderIn":0}]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"},{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Seeds","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Gene Expression Profiling"},{"majorTopic_YN":"N","descriptorName":"Gene Expression Regulation, Plant"},{"majorTopic_YN":"N","descriptorName":"Genome, Plant"},{"majorTopic_YN":"N","descriptorName":"Gene Regulatory Networks"},{"majorTopic_YN":"Y","descriptorName":"Stress, Physiological","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Droughts"},{"majorTopic_YN":"Y","descriptorName":"RNA, Long Noncoding","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"}]}}]},"keywordList":{"keyword":["Brassica napus","Drought stress","Rna-seq","Lncrna","Rewater"]},"chemicalList":{"chemical":[{"name":"RNA, Long Noncoding","registryNumber":"0"}]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1186/s12864-025-12496-8"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12870973"},{"availability":"Open 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Matin MN.","authorList":{"author":[{"fullName":"Ahmad K","firstName":"Kaleem","lastName":"Ahmad","initials":"K","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Agronomy, The University of Agriculture, Peshawar, 25130, Pakistan."}]}},{"fullName":"Munsif F","firstName":"Fazal","lastName":"Munsif","initials":"F","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Agronomy, The University of Agriculture, Peshawar, 25130, Pakistan. munsiffazal@aup.edu.pk."}]}},{"fullName":"Ullah S","firstName":"Shafi","lastName":"Ullah","initials":"S","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China."}]}},{"fullName":"Mas-Ud MA","firstName":"Md Atik","lastName":"Mas-Ud","initials":"MA","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China."}]}},{"fullName":"Ahmad I","firstName":"Ijaz","lastName":"Ahmad","initials":"I","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Agronomy, The University of Agriculture, Peshawar, 25130, Pakistan."}]}},{"fullName":"Ahmad I","firstName":"Ijaz","lastName":"Ahmad","initials":"I","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Breeding and Genetics, The University of Agriculture, Peshawar, 25130, Pakistan."}]}},{"fullName":"Ahmad M","firstName":"Masood","lastName":"Ahmad","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Horticulture, The University of Agriculture, Peshawar, 25130, Pakistan."}]}},{"fullName":"Khan MS","firstName":"Muhammad Shahid","lastName":"Khan","initials":"MS","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Plant Protection, The University of Agriculture, Peshawar, 25130, Pakistan."}]}},{"fullName":"Haq IU","firstName":"Ijaz Ul","lastName":"Haq","initials":"IU","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China."}]}},{"fullName":"Haque MA","firstName":"Md Azizul","lastName":"Haque","initials":"MA","authorId":{"type":"ORCID","value":"0000-0002-5158-4558"},"authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biotechnology, Yeungnam University, Gyeongsan, 38541, Republic of Korea. azizul@ynu.ac.kr."}]}},{"fullName":"Matin MN","firstName":"Mohammad Nurul","lastName":"Matin","initials":"MN","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Department of Biotechnology, Yeungnam University, Gyeongsan, 38541, Republic of Korea. nmatin@ru.ac.bd."}]}}]},"authorIdList":{"authorId":[{"type":"ORCID","value":"0000-0002-5158-4558"}]},"journalInfo":{"issue":"1","volume":"16","journalIssueId":4203755,"dateOfPublication":"2026 Jun","monthOfPublication":6,"yearOfPublication":2026,"printPublicationDate":"2026-06-01","journal":{"title":"Scientific reports","medlineAbbreviation":"Sci Rep","isoabbreviation":"Sci Rep","nlmid":"101563288","essn":"2045-2322","issn":"2045-2322"}},"pubYear":"2026","pageInfo":"22953","abstractText":"Drought stress poses a major challenge to sustainable agriculture, and seed priming with melatonin has shown potential to mitigate stress damage in crops. However, the effects of melatonin priming (MP) on drought tolerance in rapeseed (Brassica napus L.) remain underexplored, particularly under field conditions. In this study, two rapeseed cultivars, Shiraali and Pakola, were primed with melatonin and tested under irrigated and drought stress conditions. Results indicated significant cultivar differences for most parameters, except for plant number, peroxidase (POD), catalase (CAT), 1000-seed weight, and harvest index. MP outperformed hydro priming (HP) for most traits, except for branch number, harvest index, and seed protein content. Drought stress significantly affected all parameters, except for POD, branch number, and 1000-seed weight. Shiraali exhibited higher plant density, plant height, chlorophyll a, protein, and erucic acid content, while Pakola showed superior ascorbate peroxidase (APX) and CAT activities, chlorophyll b, carotenoids, oil content, and glucosinolates, as well as higher yield. MP enhanced antioxidant activity and pigment accumulation compared to HP. Under drought conditions, a reduction in plant pigments, yield, and protein content was observed, while oil, glucosinolates, erucic acid content, and antioxidant activities increased. Pakola outperformed Shiraali in 1000-seed weight, yield, and oil content under both irrigated and drought conditions following MP. Our results indicated that melatonin may contribute to drought tolerance through integrated physiological, biochemical, and antioxidant interactions in rapeseed.","affiliation":"Department of Agronomy, The University of Agriculture, Peshawar, 25130, Pakistan.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["Journal Article"]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Brassica napus","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug effects","majorTopic_YN":"N"},{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"},{"abbreviation":"ME","qualifierName":"metabolism","majorTopic_YN":"N"},{"abbreviation":"PH","qualifierName":"physiology","majorTopic_YN":"N"}]}},{"majorTopic_YN":"Y","descriptorName":"Seeds","meshQualifierList":{"meshQualifier":[{"abbreviation":"DE","qualifierName":"drug 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1.11.1.6"},{"name":"Antioxidants","registryNumber":"0"},{"name":"Chlorophyll","registryNumber":"1406-65-1"},{"name":"Melatonin","registryNumber":"JL5DK93RCL"}]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1038/s41598-026-56134-z"}]},"isOpenAccess":"N","inEPMC":"N","inPMC":"N","hasPDF":"N","hasBook":"N","hasSuppl":"N","citedByCount":0,"hasData":"N","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by-nc-nd","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-07-22","dateOfCreation":"2026-06-02","firstIndexDate":"2026-06-03","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-06-02","firstPublicationDate":"2026-06-02"},{"id":"42093690","source":"MED","pmid":"42093690","pmcid":"PMC13139148","fullTextIdList":{"fullTextId":["PMC13139148"]},"doi":"10.3389/fpls.2026.1729220","title":"Comparative analysis of the rhizosphere microbiome and transcriptome in clubroot-susceptible and resistant rapeseed (&lt;i&gt;Brassica napus&lt;/i&gt;).","authorString":"Liao J, Cui Y, Wang Y, Zeng X, Chen T, Xiang Y, Wang D.","authorList":{"author":[{"fullName":"Liao J","firstName":"Jingjing","lastName":"Liao","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China."}]}},{"fullName":"Cui Y","firstName":"Yuanyuan","lastName":"Cui","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China."}]}},{"fullName":"Wang Y","firstName":"Yifan","lastName":"Wang","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China."}]}},{"fullName":"Zeng X","firstName":"Xin","lastName":"Zeng","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China."}]}},{"fullName":"Chen T","firstName":"Tingting","lastName":"Chen","initials":"T","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China."}]}},{"fullName":"Xiang Y","firstName":"Yu","lastName":"Xiang","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China."}]}},{"fullName":"Wang D","firstName":"Diandong","lastName":"Wang","initials":"D","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China."}]}}]},"journalInfo":{"volume":"17","journalIssueId":4112080,"dateOfPublication":"2026 ","monthOfPublication":0,"yearOfPublication":2026,"printPublicationDate":"2026-01-01","journal":{"title":"Frontiers in plant science","medlineAbbreviation":"Front Plant Sci","isoabbreviation":"Front Plant Sci","nlmid":"101568200","essn":"1664-462X","issn":"1664-462X"}},"pubYear":"2026","pageInfo":"1729220","abstractText":"Clubroot disease, caused by <i>Plasmodiophora brassica</i>, severely threatens the rapeseed industry in China, with an annual affected area exceeding 667000 hectares. To elucidate the mechanisms in clubroot resistance, we compared the differences in soil physicochemical properties, rhizosphere microbiome, and transcriptomic responses between a susceptible variety, HYZ62 (disease index 54.86), and a resistant variety, HYZ5R (disease index 17.05), following <i>P. brassicae</i> infection. The results showed that the electrical conductivity of HYZ5R (R) was 1.73 and 1.57 times that of HYZ62 (S) in the inoculated and uninoculated treatments, respectively. Compared to the 17.18% decrease in alkali-hydrolysable nitrogen content in HYZ62 (S) after inoculation, its content in HYZ5R (R) showed no significant difference. The rhizosphere microbial community significantly differed between HYZ5R (R) and HYZ62 (S), with HYZ5R (R) exhibiting higher relative abundances of several microbial genera, such as <i>Burkholderia-Caballeronia-Paraburkholderia</i>, <i>Humibacter</i>, <i>Dyella</i>, and <i>Trichoderma</i>. Although <i>Bacillus</i> had a significantly higher relative abundance in the rhizosphere of uninoculated HYZ62 (S), its relative abundance decreased by 30.36% after infection. Transcriptome analysis revealed that, compared to HYZ62 (S), the expression of pattern-triggered immunity-related genes, such as <i>CML</i>, <i>WRKY</i>, and <i>PR1</i>, was higher in HYZ5R (R) and was more strongly induced upon inoculation. Effector-triggered immunity-related genes, such as <i>RIN4</i>, <i>RPS5</i>, and <i>HSP90</i>, were consistently expressed at higher levels. In contrast, HYZ62 (S) showed a broad suppression of defense-related gene expression after inoculation. Furthermore, although <i>P. brassicae</i> infection generally suppressed defense-related secondary metabolic pathways, including phenylpropanoid biosynthesis, the expression levels of multiple genes in this pathway remained higher in HYZ5R (R). Together, these results suggest that the higher relative abundances of specific microbial taxa in the rhizosphere and the high expression of defense-related genes are associated with the clubroot resistance in HYZ5R (R).","affiliation":"School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic-eCollection","pubTypeList":{"pubType":["research-article","Journal Article"]},"keywordList":{"keyword":["Clubroot","Transcriptome","rapeseed","Rhizosphere microorganisms","Plasmodiophora Brassicae"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3389/fpls.2026.1729220"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13139148"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13139148?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"Y","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-05-07","dateOfCreation":"2026-05-07","firstIndexDate":"2026-05-13","fullTextReceivedDate":"2026-05-20","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-04-21","firstPublicationDate":"2026-04-21"},{"id":"41829751","source":"MED","pmid":"41829751","pmcid":"PMC12986918","fullTextIdList":{"fullTextId":["PMC12986918"]},"doi":"10.3390/plants15050720","title":"Heterologous Expression of &lt;i&gt;Sorghum bicolor&lt;/i&gt; PIP1-3 Gene Improves Drought Tolerance in &lt;i&gt;Arabidopsis&lt;/i&gt; and Rapeseed.","authorString":"Gao L, Liu Y, Kang Y, Zhang Z, Xiao G.","authorList":{"author":[{"fullName":"Gao L","firstName":"Luhong","lastName":"Gao","initials":"L","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China."},{"affiliation":"Yuelushan Laboratory, Changsha 410128, China."}]}},{"fullName":"Liu Y","firstName":"Yanxin","lastName":"Liu","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China."},{"affiliation":"Yuelushan Laboratory, Changsha 410128, China."}]}},{"fullName":"Kang Y","firstName":"Yu","lastName":"Kang","initials":"Y","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China."},{"affiliation":"Yuelushan Laboratory, Changsha 410128, China."}]}},{"fullName":"Zhang Z","firstName":"Zhenqian","lastName":"Zhang","initials":"Z","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China."},{"affiliation":"Yuelushan Laboratory, Changsha 410128, China."}]}},{"fullName":"Xiao G","firstName":"Gang","lastName":"Xiao","initials":"G","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China."},{"affiliation":"Yuelushan Laboratory, Changsha 410128, China."}]}}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"5","volume":"15","journalIssueId":4149950,"dateOfPublication":"2026 Feb","monthOfPublication":2,"yearOfPublication":2026,"printPublicationDate":"2026-02-01","journal":{"title":"Plants (Basel, Switzerland)","medlineAbbreviation":"Plants (Basel)","isoabbreviation":"Plants (Basel)","nlmid":"101596181","essn":"2223-7747","issn":"2223-7747"}},"pubYear":"2026","pageInfo":"720","abstractText":"Aquaporins are key membrane proteins that mediate water transport in plants and are indispensable for maintaining cellular water homeostasis and normal physiological processes. This study investigated the function of <i>SbPIP1-3</i>, an aquaporin gene isolated from drought-tolerant Sorghum bicolor. Bioinformatics analysis, subcellular localization, and heterologous expression of <i>SbPIP1-3</i> were performed in <i>Saccharomyces cerevisiae</i>, <i>Arabidopsis thaliana</i>, and rapeseed. Sequence analysis revealed that SbPIP1-3 encodes a basic hydrophobic protein targeted to the plasma membrane, a finding further corroborated by subcellular localization assays. In yeast expression assays, <i>SbPIP1-3</i>-transformed strains retained viability under osmotic stress induced by 1.2 M mannitol, whereas non-transgenic control strains failed to survive. In <i>Arabidopsis</i> and rapeseed experiments, the <i>SbPIP1-3</i> overexpression enhanced drought tolerance (improved germination, root growth, antioxidant enzyme activity, proline content, PSII repair capacity, and survival after drought-rewatering) and reduced intracellular H<sub>2</sub>O<sub>2</sub> accumulation. Transcriptome profiling of drought-stressed transgenic <i>Arabidopsis</i> and control plants demonstrated significant upregulation of mostly stress-responsive pathways (e.g., MAPK signaling pathway and hormone signaling pathways) and key drought-tolerance genes (e.g., <i>SNRK2-2</i>, <i>SOD1</i>, <i>APX3</i>, <i>GPX3</i>, <i>P5CS1</i>). Collectively, these findings suggest that <i>SbPIP1-3</i> enhances plant drought tolerance through the following mechanisms: improving transmembrane water transport efficiency to sustain cellular osmotic balance; activating the antioxidant defense system to increase enzyme activity and mitigate reactive oxygen species (ROS) accumulation; optimizing photosynthetic protection mechanisms to preserve the structural and functional integrity of PSII; and regulating the expression of stress-responsive signaling pathways and associated functional genes.","affiliation":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic","pubTypeList":{"pubType":["research-article","Journal Article"]},"grantsList":{"grant":[{"grantId":"2022ZD04010","agency":"China government","orderIn":0}]},"keywordList":{"keyword":["Arabidopsis","Sorghum","Drought stress","Aquaporins","heterologous expression","rapeseed"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.3390/plants15050720"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12986918"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC12986918?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"Y","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"N","license":"cc by","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"Y","tmAccessionTypeList":{"accessionType":["refseq","doi"]},"dateOfCompletion":"2026-03-14","dateOfCreation":"2026-03-14","firstIndexDate":"2026-03-15","fullTextReceivedDate":"2026-03-14","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-02-27","firstPublicationDate":"2026-02-27"},{"id":"41935026","source":"MED","pmid":"41935026","pmcid":"PMC13051604","fullTextIdList":{"fullTextId":["PMC13051604"]},"doi":"10.1080/15592324.2026.2648963","title":"Advanced breeding techniques in &lt;i&gt;Brassica juncea&lt;/i&gt; L. for sustainable production under changing climate.","authorString":"Pawaiya A, Pradhan RK, Johri AK, Dua M.","authorList":{"author":[{"fullName":"Pawaiya A","firstName":"Ashita","lastName":"Pawaiya","initials":"A","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Microbial Ecology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India."}]}},{"fullName":"Pradhan RK","firstName":"Rajesh Kumar","lastName":"Pradhan","initials":"RK","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life Sciences, Jawaharlal Nehru University, New Delhi, India."}]}},{"fullName":"Johri AK","firstName":"Atul Kumar","lastName":"Johri","initials":"AK","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"School of Life Sciences, Jawaharlal Nehru University, New Delhi, India."}]}},{"fullName":"Dua M","firstName":"Meenakshi","lastName":"Dua","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Microbial Ecology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India."}]}}]},"dataLinksTagsList":{"dataLinkstag":["supporting_data"]},"journalInfo":{"issue":"1","volume":"21","journalIssueId":4076768,"dateOfPublication":"2026 Dec","monthOfPublication":12,"yearOfPublication":2026,"printPublicationDate":"2026-12-01","journal":{"title":"Plant signaling & behavior","medlineAbbreviation":"Plant Signal Behav","isoabbreviation":"Plant Signal Behav","nlmid":"101291431","essn":"1559-2324","issn":"1559-2316"}},"pubYear":"2026","pageInfo":"2648963","abstractText":"<i>Brassica juncea</i> (L.) Czern. & Coss. is an agronomically important crop cultivated worldwide as a valuable source of oil. It is a major source of edible oil in South Asia because of its high oil content, nutraceutical value, and balanced fatty acid contents. In addition to being considered a relatively hardy crop with high economic value, its productivity potential is restricted by susceptibility to various biotic and abiotic stresses, including diseases, pests, drought, heat, frost, and salinity. These constraints adversely affect yield, often leading farmers to move towards alternative crops. In this context, the use of advanced genomics, transcriptomics, and proteomics approaches can provide molecular insight into the evolutionary history, genetic diversity and adaptive response of <i>B. juncea</i> under stress and at different developmental stages. A comprehensive understanding of its molecular architecture and advanced crop improvement strategies will culminate in the development of high-yielding, stress-resistant cultivars, facilitating sustainable mustard production under a changing climate.","affiliation":"Microbial Ecology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.","publicationStatus":"ppublish","language":"eng","pubModel":"Print-Electronic","pubTypeList":{"pubType":["review-article","Review","Journal Article"]},"meshHeadingList":{"meshHeading":[{"majorTopic_YN":"Y","descriptorName":"Mustard Plant","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"},{"abbreviation":"GD","qualifierName":"growth & development","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Crops, Agricultural","meshQualifierList":{"meshQualifier":[{"abbreviation":"GE","qualifierName":"genetics","majorTopic_YN":"N"}]}},{"majorTopic_YN":"N","descriptorName":"Stress, Physiological"},{"majorTopic_YN":"Y","descriptorName":"Climate Change"},{"majorTopic_YN":"Y","descriptorName":"Plant Breeding","meshQualifierList":{"meshQualifier":[{"abbreviation":"MT","qualifierName":"methods","majorTopic_YN":"N"}]}}]},"keywordList":{"keyword":["Brassica juncea","Microbial Symbionts","Abiotic And Biotic Stresses","Crop Improvement Strategies"]},"subsetList":{"subset":[{"code":"IM","name":"Index Medicus"}]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1080/15592324.2026.2648963"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13051604"},{"availability":"Open 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fermentation: dynamic evolution of taste, aroma, and microbial communities.","authorString":"Zhang F, Huang X, Aaqil M, Huang R, Yao J, Zhao C.","authorList":{"author":[{"fullName":"Zhang F","firstName":"Feng","lastName":"Zhang","initials":"F","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China."}]}},{"fullName":"Huang X","firstName":"Xiaolin","lastName":"Huang","initials":"X","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China."}]}},{"fullName":"Aaqil M","firstName":"Muhammad","lastName":"Aaqil","initials":"M","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China."}]}},{"fullName":"Huang R","firstName":"Renwang","lastName":"Huang","initials":"R","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Luoping Fuxin Agricultural Development Company Limited, Luoping 655800, China."}]}},{"fullName":"Yao J","firstName":"Jiawen","lastName":"Yao","initials":"J","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"Luoping Fuxin Agricultural Development Company Limited, Luoping 655800, China."}]}},{"fullName":"Zhao C","firstName":"Cunchao","lastName":"Zhao","initials":"C","authorAffiliationDetailsList":{"authorAffiliation":[{"affiliation":"College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China."},{"affiliation":"Yunnan Plateau Characteristic Agricultural Industry Research Institute, Kunming 650201, China."},{"affiliation":"Yunnan International Joint Laboratory of China-Cambodia Signiture Agro-Products Green Development, Kunming 650201, China."}]}}]},"dataLinksTagsList":{"dataLinkstag":["altmetrics"]},"journalInfo":{"volume":"36","journalIssueId":4187235,"dateOfPublication":"2026 May","monthOfPublication":5,"yearOfPublication":2026,"printPublicationDate":"2026-05-01","journal":{"title":"Food chemistry: X","medlineAbbreviation":"Food Chem X","isoabbreviation":"Food Chem X","nlmid":"101751436","essn":"2590-1575","issn":"2590-1575"}},"pubYear":"2026","pageInfo":"104046","abstractText":"Rapeseed meal is rich in protein, but its high-value utilization remains insufficient. In this study, high-temperature steam pretreatment (HTSP) was applied to rapeseed meal to reduce safety- and flavor-limiting factors and improve its suitability as a raw material for soy sauce fermentation. Compared with the CON group, HTSP significantly reduced the contents of glucosinolates, isothiocyanates, erucic acid, and phenolic-related compounds in rapeseed meal, with glucosinolates and isothiocyanates decreasing by 59.02% and 92.65%, respectively. Meanwhile, the contents of protein, soluble protein, and reducing sugars increased to 39.28, 16.42, and 1.18 g/100 g, corresponding to 1.11-, 2.01-, and 1.97-fold those of the CON group, respectively. Subsequently, HTSP-treated rapeseed meal was used as the main raw material for soy sauce fermentation. During the 1-15 d fermentation period, the sample fermented for 15 d showed the most favorable physicochemical and flavor characteristics. At this stage, the contents of total nitrogen, amino acid nitrogen, reducing sugars, total acidity, and soluble solids reached 1.16, 0.70, 2.13, 1.22, and 34.31 g/100 mL, corresponding to 4.30-, 3.33-, 3.23-, 30.50-, and 4.73-fold those on day 1, respectively. During fermentation, the taste profile shifted from bitterness and sourness toward sweetness and umami, while the aroma profile evolved from fruity notes to nutty, roasted, and floral notes. Correlation analysis indicated that <i>Weissella</i> and <i>Zygosaccharomyces rouxii</i> were important microbial taxa closely associated with the quality and flavor development of rapeseed meal soy sauce, and may contribute to the accumulation of taste compounds in the early stage and aroma formation in the middle and late stages, respectively. Overall, HTSP effectively enhanced the utilization potential of rapeseed meal as a fermentation substrate, supporting its high-value application in soy sauce brewing.","affiliation":"College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.","publicationStatus":"epublish","language":"eng","pubModel":"Electronic-eCollection","pubTypeList":{"pubType":["research-article","Journal Article"]},"keywordList":{"keyword":["Soy sauce","Rapeseed meal","Zygosaccharomyces Rouxii","Weissella","High-Temperature Steam Pretreatment"]},"fullTextUrlList":{"fullTextUrl":[{"availability":"Subscription required","availabilityCode":"S","documentStyle":"doi","site":"DOI","url":"https://doi.org/10.1016/j.fochx.2026.104046"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"html","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13231012"},{"availability":"Open access","availabilityCode":"OA","documentStyle":"pdf","site":"Europe_PMC","url":"https://europepmc.org/articles/PMC13231012?pdf=render"}]},"isOpenAccess":"Y","inEPMC":"Y","inPMC":"Y","hasPDF":"Y","hasBook":"N","hasSuppl":"Y","citedByCount":0,"hasData":"Y","hasReferences":"Y","hasTextMinedTerms":"Y","hasDbCrossReferences":"N","hasLabsLinks":"Y","license":"cc by-nc-nd","hasEvaluations":"N","authMan":"N","epmcAuthMan":"N","nihAuthMan":"N","hasTMAccessionNumbers":"N","dateOfCompletion":"2026-06-05","dateOfCreation":"2026-06-05","firstIndexDate":"2026-06-06","fullTextReceivedDate":"2026-06-04","dateOfRevision":"2026-08-13","electronicPublicationDate":"2026-05-28","firstPublicationDate":"2026-05-28"}]}}